Stabilized coronavirus spikes are resistant to conformational changes induced by receptor recognition or proteolysis
Explore this paper's citation graph
Summary
Cryo-EM analyses of a stabilized trimeric SARS-CoV S, as well as the trypsin-cleaved, stabilized S, and its interactions with ACE2 are presented, finding that neither binding to ACE2 nor cleavage bytrypsin at the S1/S2 cleavage site impart large conformational changes within stabilized SARV S.
- Type
- article
- Published
- 2018-10-24
- Cited by
- 480
- References
- 52
- Access
- Open access
- OpenAlex
- https://openalex.org/W2896109093
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:53022573
Keywords
Coronavirus, Proteases, Viral entry, Cleavage (geology), Conformational change
References
- SARS Immunity and Vaccination.
- Activation of the SARS coronavirus spike protein via sequential proteolytic cleavage at two distinct sites
- Angiotensin-converting enzyme 2 is a functional receptor for the SARS coronavirus
- Stabilization of the Soluble, Cleaved, Trimeric Form of the Envelope Glycoprotein Complex of Human Immunodeficiency Virus Type 1
- Receptor-bound porcine epidemic diarrhea virus spike protein cleaved by trypsin induces membrane fusion
- Structure of influenza haemagglutinin at the pH of membrane fusion
- Structure of SARS Coronavirus Spike Receptor-Binding Domain Complexed with Receptor
- Host cell proteases: critical determinants of coronavirus tropism and pathogenesis
- Inhibitors of cathepsin L prevent severe acute respiratory syndrome coronavirus entry.
- DoG Picker and TiltPicker: software tools to facilitate particle selection in single particle electron microscopy
- Vesicular stomatitis virus pseudotyped with severe acute respiratory syndrome coronavirus spike protein.
- Molecular architecture of native HIV-1 gp 120 trimers
- 310 helices in channels and other membrane proteins
- Automated molecular microscopy: the new Leginon system.
- Views of helical peptides: a proposal for the position of 3(10)-helix along the thermodynamic folding pathway.
- A Next-Generation Cleaved, Soluble HIV-1 Env Trimer, BG505 SOSIP.664 gp140, Expresses Multiple Epitopes for Broadly Neutralizing but Not Non-Neutralizing Antibodies
- Atomic accuracy models from 4.5 Å cryo-electron microscopy data with density-guided iterative local refinement
- Structural Characterization of Cleaved, Soluble HIV-1 Envelope Glycoprotein Trimers
- The Coronavirus Spike Protein Is a Class I Virus Fusion Protein: Structural and Functional Characterization of the Fusion Core Complex
- N-Terminal Domain of the Murine Coronavirus Receptor CEACAM1 Is Responsible for Fusogenic Activation and Conformational Changes of the Spike Protein
Cited by
- Effect of Cysteine Oxidation in SARS-CoV-2 Receptor-Binding Domain on Its Interaction with Two Cell Receptors: Insights from Atomistic Simulations
- Structural basis for human coronavirus attachment to sialic acid receptors
- Antibodies and vaccines against Middle East respiratory syndrome coronavirus
- Biochemical Analysis of Coronavirus Spike Glycoprotein Conformational Intermediates during Membrane Fusion
- Designed nanoparticles elicit cross-reactive antibody responses to conserved influenza virus hemagglutinin stem epitopes
- Glycine 29 Is Critical for Conformational Changes of the Spike Glycoprotein of Mouse Hepatitis Virus A59 Triggered by either Receptor Binding or High pH
- The 3.1-Angstrom Cryo-electron Microscopy Structure of the Porcine Epidemic Diarrhea Virus Spike Protein in the Prefusion Conformation
- The human coronavirus HCoV-229E S-protein structure and receptor binding
- Structures of MERS-CoV spike glycoprotein in complex with sialoside attachment receptors
- Cryo-EM Structure of the 2019-nCoV Spike in the Prefusion Conformation
- Structural genomics and interactomics of 2019 Wuhan novel coronavirus, 2019-nCoV, indicate evolutionary conserved functional regions of viral proteins
- Structure and immune recognition of the porcine epidemic diarrhea virus spike protein
- Structure, Function, and Antigenicity of the SARS-CoV-2 Spike Glycoprotein
- Structure of mouse coronavirus spike protein complexed with receptor reveals mechanism for viral entry
- Perspectives on monoclonal antibody therapy as potential therapeutic intervention for Coronavirus disease-19 (COVID-19).
- Structural basis for the recognition of SARS-CoV-2 by full-length human ACE2
- Cryo-electron microscopy structure of the SADS-CoV spike glycoprotein provides insights into an evolution of unique coronavirus spike proteins
- Spontaneous Hinge-Bending Motions of Angiotensin I Converting Enzyme: Role in Activation and Inhibition
- Cross-reactive antibody response between SARS-CoV-2 and SARS-CoV infections
- Structural Genomics of SARS-CoV-2 Indicates Evolutionary Conserved Functional Regions of Viral Proteins
Related papers
- A novel system to study adenovirus tropism to normal and malignant colon tissues.
- Causal relationships between HIV-1 coreceptor utilization, tropism, and pathogenesis in human thymus.
- Advance in Molecular Mechanism of Infectious Bronchitis Virus Infection
- Determination of the factors responsible for host tropism of SARS-CoV-2-related bat coronaviruses
- A Neonatal Murine Model for Caprine Enterovirus Infection and the Viral Tissue Tropism
- Definition of the range and distribution of human immunodeficiency virus macrophage tropism using PCR-based infectivity measurements.
- Genotypic Prediction of Tropism of Highly Diverse HIV-1 Strains from Cameroon
- Analysis of HIV-1 cell tropism in patients receiving HAART
- The impact of hepatitis C virus entry on viral tropism