DoG Picker and TiltPicker: software tools to facilitate particle selection in single particle electron microscopy
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Summary
This work presents two software tools meant to facilitate particle picking, an early stage in the single-particle processing of unknown macromolecules, and TiltPicker, an interactive graphical interface application designed to streamline the selection of particle pairs from tilted-pair datasets.
- Type
- article
- Published
- 2009-05-01
- Cited by
- 601
- References
- 28
- Access
- Open access
- OpenAlex
- https://openalex.org/W2028470175
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:10625226
Keywords
Particle (ecology), sort, Computer science, Software, Selection (genetic algorithm)
References
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- Scale-Space Theory : A Basic Tool for Analysing Structures at Different Scales
- SPIDER and WEB: processing and visualization of images in 3D electron microscopy and related fields.
- A test-bed for optimizing high-resolution single particle reconstructions.
- The orthogonal tilt reconstruction method: an approach to generating single-class volumes with no missing cone for ab initio reconstruction of asymmetric particles.
- Automated molecular microscopy: the new Leginon system.
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- Immunization-elicited Broadly Protective Antibody Reveals Ebolavirus Fusion Loop as a Site of Vulnerability
- Visualizing molecular machines in action: Single-particle analysis with structural variability.
- Single-particle electron microscopy of animal fatty acid synthase describing macromolecular rearrangements that enable catalysis.
- The orthogonal tilt reconstruction method.
- Protective mAbs and Cross-Reactive mAbs Raised by Immunization with Engineered Marburg Virus GPs
- Conformational dynamics of large proteins and protein complexes.
- Architecture of the Complex formed by Large and Small Terminase Subunits from Bacteriophage P22
- Structural studies of allosteric regulation in the class Ia Ribonucleotide reductase from Escherichia coli
- Molecular Basis of the Rapamycin Insensitivity of Target Of Rapamycin Complex 2.
- Structural constraints determine the glycosylation of HIV-1 envelope trimers
- Automatic approaches for microscopy imaging based on machine learning and spatial statistics
- Towards a structural understanding of adeno- associated virus serotype 2 and its recognition by antibodies
- Chemical Cross-Linking Stabilizes Native-Like HIV-1 Envelope Glycoprotein Trimer Antigens
- A network of SMG-8, SMG-9 and SMG-1 C-terminal insertion domain regulates UPF1 substrate recruitment and phosphorylation
- Model building and refinement of a natively glycosylated HIV-1 Env protein by high-resolution cryoEM
- Design and structure of two HIV-1 clade C SOSIP.664 trimers that increase the arsenal of native-like Env immunogens
- An optimized locally adaptive non-local means denoising filter for cryo-electron microscopy data.
- Structure of an RNA Silencing Complex of the CRISPR-Cas immune system
- Single-particle EM reveals plasticity of interactions between the adenovirus penton base and integrin αVβ3
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