Cryo-EM Structure of the 2019-nCoV Spike in the Prefusion Conformation
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Summary
The authors show that this protein binds at least 10 times more tightly than the corresponding spike protein of severe acute respiratory syndrome (SARS)–CoV to their common host cell receptor, and test several published SARS-CoV RBD-specific monoclonal antibodies found that they do not have appreciable binding to 2019-nCoV S, suggesting that antibody cross-reactivity may be limited between the two RBDs.
- Type
- preprint
- Published
- 2020-02-15
- Cited by
- 8,381
- References
- 53
- Access
- Open access
- OpenAlex
- https://openalex.org/W3005605085
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:211215068
Keywords
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), Trimer, Coronavirus disease 2019 (COVID-19), Betacoronavirus, 2019-20 coronavirus outbreak
References
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- Collaboration gets the most out of software
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- Cathepsin L Functionally Cleaves the Severe Acute Respiratory Syndrome Coronavirus Class I Fusion Protein Upstream of Rather than Adjacent to the Fusion Peptide
- Prefusion structure of a human coronavirus spike protein
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- Cryo-electron microscopy structures of the SARS-CoV spike glycoprotein reveal a prerequisite conformational state for receptor binding
- Cryo-EM structures of MERS-CoV and SARS-CoV spike glycoproteins reveal the dynamic receptor binding domains
- Immunogenicity and structures of a rationally designed prefusion MERS-CoV spike antigen
- cisTEM: User-friendly software for single-particle image processing
- cisTEM, user-friendly software for single-particle image processing
Cited by
- A Cryptic Site of Vulnerability on the Receptor Binding Domain of the SARS-CoV-2 Spike Glycoprotein
- HVIDB: a comprehensive database for human-virus protein-protein interactions
- Effect of Cysteine Oxidation in SARS-CoV-2 Receptor-Binding Domain on Its Interaction with Two Cell Receptors: Insights from Atomistic Simulations
- Pyroptotic cell death in SARS-CoV-2 infection: revealing its roles during the immunopathogenesis of COVID-19
- Predicting the receptor-binding domain usage of the coronavirus based on kmer frequency on spike protein
- Non-uniform refinement: adaptive regularization improves single-particle cryo-EM reconstruction
- Single-cell RNA expression profiling of ACE2, the putative receptor of Wuhan 2019-nCov
- Interpretable detection of novel human viruses from genome sequencing data
- Preliminary Identification of Potential Vaccine Targets for the COVID-19 Coronavirus (SARS-CoV-2) Based on SARS-CoV Immunological Studies
- Structure of dimeric full-length human ACE2 in complex with B0AT1
- Structural basis for the recognition of the 2019-nCoV by human ACE2
- Spike protein binding prediction with neutralizing antibodies of SARS-CoV-2
- Molecular mechanism of evolution and human infection with the novel coronavirus (2019-nCoV)
- The SARS-CoV-2 receptor ACE2 expression of maternal-fetal interface and fetal organs by single cell transcriptome study
- Functional pangenome analysis suggests inhibition of the protein E as a readily available therapy for COVID-2019
- TWIRLS, an automated topic-wise inference method based on massive literature, suggests a possible mechanism via ACE2 for the pathological changes in the human host after coronavirus infection
- Mutations, Recombination and Insertion in the Evolution of 2019-nCoV
- Crystal structure of the 2019-nCoV spike receptor-binding domain bound with the ACE2 receptor
- Understanding of COVID‐19 based on current evidence
- Potential T-cell and B-cell Epitopes of 2019-nCoV
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