The new frontier of genome engineering with CRISPR-Cas9
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Summary
The power of the CRISPR-Cas9 technology to systematically analyze gene functions in mammalian cells, study genomic rearrangements and the progression of cancers or other diseases, and potentially correct genetic mutations responsible for inherited disorders is illustrated.
- Type
- review
- Published
- 2014-11-28
- Cited by
- 6,144
- References
- 161
- OpenAlex
- https://openalex.org/W2050480410
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:6299381
Keywords
CRISPR, Cas9, Genome editing, Biology, Computational biology
References
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- Targeted chromosomal cleavage and mutagenesis in Drosophila using zinc-finger nucleases.
- Simple and efficient CRISPR/Cas9-mediated targeted mutagenesis in Xenopus tropicalis
- Genetic and physical analysis of double-strand break repair and recombination in Saccharomyces cerevisiae.
- Site-specific recombination determined by I-SceI, a mitochondrial group I intron-encoded endonuclease expressed in the yeast nucleus.
- RNA-guided genome editing in plants using a CRISPR-Cas system.
- Harnessing the CRISPR/Cas9 system to disrupt latent HIV-1 provirus
- Peptide nucleic acid-targeted mutagenesis of a chromosomal gene in mouse cells.
Cited by
- Targeted Mutagenesis, Precise Gene Editing, and Site-Specific Gene Insertion in Maize Using Cas9 and Guide RNA[OPEN]
- Neurobehavioral Development of Common Marmoset Monkeys
- Conservation Biological Control of Pests in the Molecular Era: New Opportunities to Address Old Constraints
- Interspecies chimeric complementation for the generation of functional human tissues and organs in large animal hosts
- A dual AAV system enables the Cas9-mediated correction of a metabolic liver disease in newborn mice
- Proteolytic activation defines distinct lymphangiogenic mechanisms for VEGFC and VEGFD.
- A Comprehensive Toolbox for Genome Editing in Cultured Drosophila melanogaster Cells
- mCAL: A New Approach for Versatile Multiplex Action of Cas9 Using One sgRNA and Loci Flanked by a Programmed Target Sequence
- Deciphering Combinatorial Genetics.
- LiPS-A3S, a human genomic site for robust expression of inserted transgenes
- PPR-SMR protein SOT1 has RNA endonuclease activity
- Delivery technologies for genome editing
- Going non-viral: the Sleeping Beauty transposon system breaks on through to the clinical side
- A CRISPR New World: Attitudes in the Public toward Innovations in Human Genetic Modification
- Ovary as a Biomarker of Health and Longevity: Insights from Genetics
- Gene editing tools: state-of-the-art and the road ahead for the model and non-model fishes
- Development of a versatile and conventional technique for gene disruption in filamentous fungi based on CRISPR-Cas9 technology
- Lissencephaly-1 dependent axonal retrograde transport of L1-type CAM Neuroglian in the adult drosophila central nervous system
- Molecular tools for gene manipulation in filamentous fungi
- Method for Multiplexing CRISPR/Cas9 in Saccharomyces cerevisiae Using Artificial Target DNA Sequences
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