RNA-guided genome editing in plants using a CRISPR-Cas system.
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Summary
The results demonstrate that the CRISPR-Cas system can be exploited as a powerful tool for gene targeting and precise genome editing in plants and suggests that mismatch position between gRNA seed and target DNA is an important determinant of the gRNA-Cas9 targeting specificity.
- Type
- article
- Published
- 2013-11-01
- Cited by
- 736
- References
- 29
- Access
- Open access
- OpenAlex
- https://openalex.org/W1963554509
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:25818793
Keywords
Biology, CRISPR, Genome editing, Computational biology, RNA
References
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- Multiplex Genome Engineering Using CRISPR/Cas Systems
- A Simple Cipher Governs DNA Recognition by TAL Effectors
- Generation of gene-modified mice via Cas9/RNA-mediated gene targeting
- High frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells
- Targeted Genome Editing Across Species Using ZFNs and TALENs
- Disease Resistance and Abiotic Stress Tolerance in Rice Are Inversely Modulated by an Abscisic Acid–Inducible Mitogen-Activated Protein Kinase Online version contains Web-only data. Article, publication date, and citation information can be found at www.plantcell.org/cgi/doi/10.1105/tpc.008714.
- Genome engineering in Saccharomyces cerevisiae using CRISPR-Cas systems
Cited by
- CRISPR/Cas9-mediated targeted mutagenesis in the liverwort Marchantia polymorpha L.
- Efficient targeted multiallelic mutagenesis in tetraploid potato (Solanum tuberosum) by transient CRISPR-Cas9 expression in protoplasts
- A simple and efficient CRISPR/Cas9 platform for induction of single and multiple, heritable mutations in barley (Hordeum vulgare L.)
- In vitro Enzymology of Cas9
- Parameters affecting frequency of CRISPR/Cas9 mediated targeted mutagenesis in rice
- Biallelic genome modification in F0 Xenopus tropicalis embryos using the CRISPR/Cas system
- Targeted mutagenesis in soybean using the CRISPR-Cas9 system
- Tomato I2 immune receptor can be engineered to confer partial resistance to the oomycete Phytophthora infestans in addition to the fungus Fusarium oxysporum
- A detailed procedure for CRISPR/Cas9-mediated gene editing in Arabidopsis thaliana
- Research progress of genome editing and derivative technologies in plants.
- DNA damage and repair in plants – from models to crops
- Comparison of CRISPR/Cas9 expression constructs for efficient targeted mutagenesis in rice
- CRISPR/Cas9-Mediated Genome Editing in Soybean Hairy Roots
- CRISPR-P: a web tool for synthetic single-guide RNA design of CRISPR-system in plants.
- Application of genomics-assisted breeding for generation of climate resilient crops: progress and prospects
- Modification of the PthA4 effector binding elements in Type I CsLOB1 promoter using Cas9/sgRNA to produce transgenic Duncan grapefruit alleviating XccΔpthA4:dCsLOB1.3 infection.
- Egg cell-specific promoter-controlled CRISPR/Cas9 efficiently generates homozygous mutants for multiple target genes in Arabidopsis in a single generation
- RNA-Guided Nucleases: A New Era for Engineering the Genomes of Model and Nonmodel Organisms
- Genome editing in rice and wheat using the CRISPR/Cas system
- DNA interrogation by the CRISPR RNA-guided endonuclease Cas9
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