Inducible Genome Editing with Conditional CRISPR/Cas9 Mice
Explore this paper's citation graph
Summary
The generation of a transgenic mouse harboring a doxycycline-regulated Cas9 allele for inducible genome engineering provides a flexible platform for genome engineering since editing is achieved by exogenous delivery of sgRNAs and should allow for the modeling of a range of biological and pathological processes.
- Type
- article
- Published
- 2018-03-08
- Cited by
- 17
- References
- 29
- Access
- Open access
- OpenAlex
- https://openalex.org/W2790778573
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:3809741
Keywords
CRISPR, Genome editing, Cas9, Computational biology, Biology
References
- Protospacer Adjacent Motif (PAM)-Distal Sequences Engage CRISPR Cas9 DNA Target Cleavage
- A CRISPR/Cas9 Functional Screen Identifies Rare Tumor Suppressors
- Expanding the Biologist's Toolkit with CRISPR-Cas9.
- Methods for studying pro- and antiapoptotic genes in nonimmortal cells.
- Targeting Synthetic Lethal Interactions between Myc and the eIF4F Complex Impedes Tumorigenesis
- CRISPR-Cas9 Knockin Mice for Genome Editing and Cancer Modeling
- A tumour suppressor network relying on the polyamine–hypusine axis
- ‘Green mice’ as a source of ubiquitous green cells
- Translation initiation factor eIF4F modifies the dexamethasone response in multiple myeloma
- Conditional Reverse Tet-Transactivator Mouse Strains for the Efficient Induction of TRE-Regulated Transgenes in Mice
- Growth inhibition and DNA damage induced by Cre recombinase in mammalian cells
- Efficient method to generate single‐copy transgenic mice by site‐specific integration in embryonic stem cells
- The E mu-myc transgenic mouse. A model for high-incidence spontaneous lymphoma and leukemia of early B cells
- Nondefective spleen necrosis virus-derived vectors define the upper size limit for packaging reticuloendotheliosis viruses.
- Efficient Delivery of Genome-Editing Proteins In Vitro and In Vivo
- Inducible in vivo genome editing with CRISPR/Cas9
- The pCL vector system: rapid production of helper-free, high-titer, recombinant retroviruses
- Reversible suppression of an essential gene in adult mice using transgenic RNA interference
- Dissecting eIF4E action in tumorigenesis.
- Molecular Cloning: A Laboratory Manual
Cited by
- A Tet/Q Hybrid System for Robust and Versatile Control of Transgene Expression in C. elegans
- Chemical Biology Gateways to Mapping Location, Association, and Pathway Responsivity
- CRISPR Genome Editing: A Technical & Policy Primer
- Engineering CRISPR mouse models of cancer.
- Animal Models to Study Cancer and Its Microenvironment.
- ObLiGaRe doxycycline Inducible (ODIn) Cas9 system driving pre-clinical drug discovery, from design to cancer treatment
- Development of an ObLiGaRe Doxycycline Inducible Cas9 system for pre-clinical cancer drug discovery
- Drug discovery oncology in a mouse: concepts, models and limitations
- Forward and Reverse Genetics of B Cell Malignancies: From Insertional Mutagenesis to CRISPR-Cas
- CRISPR somatic genome engineering and cancer modeling in the mouse pancreas and liver
- The double mutations of acvr2aa and acvr2ba leads to muscle hypertrophy in zebrafish
- Endothelial mechanobiology
- A CRISPR View of Hematopoietic Stem Cells: Moving Innovative Bioengineering into the Clinic
- Tutorial: design and execution of CRISPR in vivo screens
- Massively parallel in vivo Perturb-seq screening
- Development and characterisation of improved unifocal primary mouse lung cancer models with metastatic potential
- Stage- and tissue-specific gene editing using 4-OHT inducible Cas9 in whole organism
- Stage- and tissue-specific gene editing using 4-OHT–inducible Cas9 in whole organism
Related papers
- Temperature effect on CRISPR-Cas9 mediated genome editing.
- CRISPR-Cas9 system: A new-fangled dawn in gene editing.
- CRISPR/Cas9 system: A promising technology for the treatment of inherited and neoplastic hematological diseases
- Therapeutic applications of CRISPR RNA-guided genome editing.
- CRISPR/Cas9-Based Genome Editing for Disease Modeling and Therapy: Challenges and Opportunities for Nonviral Delivery.
- Efficient CRISPR/Cas9 genome editing with low off-target effects in zebrafish
- Chemical transformation mediated CRISPR/Cas9 genome editing in Escherichia coli
- CRISPR/Cas in genome defense and gene editing
- CRISPR/Cas9 Platforms for Genome Editing in Plants: Developments and Applications.