Expanding the Biologist's Toolkit with CRISPR-Cas9.
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Summary
This work highlights the recent explosion in visionary applications of CRISPR-Cas9 that promises to usher in a new era of biological understanding and control.
- Type
- review
- Published
- 2015-05-21
- Cited by
- 404
- References
- 89
- Access
- Open access
- OpenAlex
- https://openalex.org/W1600677671
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:206991714
Keywords
CRISPR, Cas9, Biology, Genome editing, Function (biology)
References
- Genome-wide binding of the CRISPR endonuclease Cas9 in mammalian cells
- Protospacer Adjacent Motif (PAM)-Distal Sequences Engage CRISPR Cas9 DNA Target Cleavage
- Genetic screens in human cells using the CRISPR/Cas9 system
- Proteins and DNA elements essential for the CRISPR adaptation process in Escherichia coli
- DNA interrogation by the CRISPR RNA-guided endonuclease Cas9
- CRISPR-Cas9 Knockin Mice for Genome Editing and Cancer Modeling
- In vivo engineering of oncogenic chromosomal rearrangements with the CRISPR/Cas9 system
- Genome-Scale CRISPR-Cas9 Knockout Screening in Human Cells
- Low incidence of off-target mutations in individual CRISPR-Cas9 and TALEN targeted human stem cell clones detected by whole-genome sequencing
- Efficient In Vivo Genome Editing Using RNA-Guided Nucleases
- Simultaneous editing of three homoeoalleles in hexaploid bread wheat confers heritable resistance to powdery mildew
- Fusion of catalytically inactive Cas9 to FokI nuclease improves the specificity of genome modification
- High-throughput screening of a CRISPR/Cas9 library for functional genomics in human cells
- CRISPR elements in Yersinia pestis acquire new repeats by preferential uptake of bacteriophage DNA, and provide additional tools for evolutionary studies.
- Structural basis of PAM-dependent target DNA recognition by the Cas9 endonuclease
- Intervening Sequences of Regularly Spaced Prokaryotic Repeats Derive from Foreign Genetic Elements
- Regulating gene drives
- Synthesizing AND gate genetic circuits based on CRISPR-Cas9 for identification of bladder cancer cells
- Chromosomal deletions and inversions mediated by TALENs and CRISPR/Cas in zebrafish
- CRISPR-assisted editing of bacterial genomes
Cited by
- Lentivirus pre-packed with Cas9 protein for safer gene editing
- Structural basis of CRISPR–SpyCas9 inhibition by an anti-CRISPR protein
- A CRISPR New World: Attitudes in the Public toward Innovations in Human Genetic Modification
- Next-Generation Sequencing of Genome-Wide CRISPR Screens
- Synaptic-vesicle fusion: a need for speed
- Bacterial CRISPR: Accomplishments and Prospects
- Presynaptic NMDA receptors: Roles and rules.
- Isolation and Characterisation of Relaxed Specificity I-TevI Nuclease Domain Mutants
- Efficient Genome Editing in Caenorhabditis elegans with a Toolkit of Dual-Marker Selection Cassettes
- CASFISH: CRISPR/Cas9-mediated in situ labeling of genomic loci in fixed cells
- Efficient Multiplexed Integration of Synergistic Alleles and Metabolic Pathways in Yeasts via CRISPR-Cas.
- Harnessing Type I and Type III CRISPR-Cas systems for genome editing
- Diversity of CRISPR-Cas immune systems and molecular machines
- Genetic engineering of modular PKSs: from combinatorial biosynthesis to synthetic biology.
- Conformational control of DNA target cleavage by CRISPR–Cas9
- New Vectors for Simple and Streamlined CRISPR-Cas9 Genome Editing in Saccharomyces cerevisiae
- The genetics of preterm birth: Progress and promise.
- A Scalable Genome-Editing-Based Approach for Mapping Multiprotein Complexes in Human Cells.
- Resources for the design of CRISPR gene editing experiments
- Rapid characterization of CRISPR-Cas9 protospacer adjacent motif sequence elements
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