CRISPR-assisted editing of bacterial genomes
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Summary
The exhaustively analyze dual-RNA:Cas9 target requirements to define the range of targetable sequences and show strategies for editing sites that do not meet these requirements, suggesting the versatility of this technique for bacterial genome engineering.
- Type
- article
- Published
- 2013-01-29
- Cited by
- 1,848
- References
- 53
- OpenAlex
- https://openalex.org/W2000046950
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:88777201
Keywords
CRISPR, Cas9, Trans-activating crRNA, Biology, Recombineering
References
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Cited by
- Multiplex and homologous recombination-mediated plant genome editing via guide RNA/Cas9
- Genetic correction using engineered nucleases for gene therapy applications
- CRISPR-Cas Immunity against Phages: Its Effects on the Evolution and Survival of Bacterial Pathogens
- Protospacer Adjacent Motif (PAM)-Distal Sequences Engage CRISPR Cas9 DNA Target Cleavage
- Beyond traditional pharmacology: new tools and approaches
- mCAL: A New Approach for Versatile Multiplex Action of Cas9 Using One sgRNA and Loci Flanked by a Programmed Target Sequence
- CRISPR/Cas9-Based Efficient Genome Editing in Clostridium ljungdahlii, an Autotrophic Gas-Fermenting Bacterium.
- One step engineering of the small-subunit ribosomal RNA using CRISPR/Cas9
- CRISPR/Cas-Mediated Site-Specific Mutagenesis in Arabidopsis thaliana Using Cas9 Nucleases and Paired Nickases.
- Deciphering Combinatorial Genetics.
- Investigation Into the Effects of Nucleotide Content on the Electrical Characteristics of DNA Plasmid Molecular Wires
- CRISPR-Cas Genome Surgery in Ophthalmology
- Enabling tools for high-throughput detection of metabolites: Metabolic engineering and directed evolution applications.
- Microbial Electrosynthesis I: Pure and Defined Mixed Culture Engineering.
- Method for Multiplexing CRISPR/Cas9 in Saccharomyces cerevisiae Using Artificial Target DNA Sequences
- Programmable RNA cleavage and recognition by a natural CRISPR-Cas9 system from Neisseria meningitidis
- Developing a Synthetic Biology Toolkit for Comamonas testosteroni, an Emerging Cellular Chassis for Bioremediation.
- Multiplex navigation of global regulatory networks (MINR) in yeast for improved ethanol tolerance and production.
- CRISPR–Cas9 in genome editing: Its function and medical applications
- An undergraduate laboratory experience using CRISPR‐cas9 technology to deactivate green fluorescent protein expression in Escherichia coli
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