Structural basis of PAM-dependent target DNA recognition by the Cas9 endonuclease
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Summary
A crystal structure of Streptococcus pyogenes Cas9 in complex with a single-molecule guide RNA and a target DNA containing a canonical 5′-NGG-3′ PAM is reported, revealing that the PAM motif resides in a base-paired DNA duplex.
- Type
- article
- Published
- 2014-07-27
- Cited by
- 1,252
- References
- 37
- OpenAlex
- https://openalex.org/W1988101725
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:4469497
Keywords
Cas9, DNA, RNA, Nuclease, Endonuclease
References
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- Cas9 as a versatile tool for engineering biology
- CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in Eukaryotes
- Crystal Structure of Cas9 in Complex with Guide RNA and Target DNA
- The Streptococcus thermophilus CRISPR/Cas system provides immunity in Escherichia coli
- CRISPR RNA maturation by trans-encoded small RNA and host factor RNase III
- Programmable repression and activation of bacterial gene expression using an engineered CRISPR-Cas system
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- CRISPR–Cas9 in genome editing: Its function and medical applications
- Single-molecule FRET studies of Cas9 endonuclease.
- Structure and Dynamics of the CRISPR-Cas9 Catalytic Complex
- Recent advances in structural studies of the CRISPR-Cas-mediated genome editing tools
- In vitro Enzymology of Cas9
- Efficient Multiple Genome Modifications Induced by the crRNAs, tracrRNA and Cas9 Protein Complex in Zebrafish
- Efficient generation of hiPSC neural lineage specific knockin reporters using the CRISPR/Cas9 and Cas9 double nickase system.
- Applications of CRISPR-Cas9 mediated genome engineering
- Targeted mutagenesis in soybean using the CRISPR-Cas9 system
- A conserved structural chassis for mounting versatile CRISPR RNA-guided immune responses
- Mechanism of CRISPR-RNA guided recognition of DNA targets in Escherichia coli
- The Bacterial Origins of the CRISPR Genome-Editing Revolution
- Expanding the Biologist's Toolkit with CRISPR-Cas9.
- CRISPR-Cas9 Based Genome Engineering: Opportunities in Agri-Food-Nutrition and Healthcare
- Pre‐organized Guide RNA in the Cas9 Complex Is Ready for the Selection of Target Double‐Stranded DNA
- Organische Chemie 2014
- DSSR: an integrated software tool for dissecting the spatial structure of RNA
- Minimizing off-Target Mutagenesis Risks Caused by Programmable Nucleases
- Putting RNA to work: Translating RNA fundamentals into biotechnological engineering practice.
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