Genome Editing: CRISPR-Cas9.
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Summary
The protocols and guidelines to facilitate implementation of CRISPR-Cas9 technology in fungi where few or no genetic tools are in place are presented and how to identify dominant markers for genetic transformation is explained.
- Type
- article
- Published
- 2018-01-01
- Cited by
- 16
- References
- 23
- OpenAlex
- https://openalex.org/W2806961641
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:265716504
Keywords
CRISPR, Genome editing, Cas9, Biology, Subgenomic mRNA
References
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- A Programmable Dual-RNA–Guided DNA Endonuclease in Adaptive Bacterial Immunity
- The new frontier of genome engineering with CRISPR-Cas9
- Gene targeting in filamentous fungi: the benefits of impaired repair
- SLiCE: a novel bacterial cell extract-based DNA cloning method
- A Method for Gene Disruption That Allows Repeated Use of URA3 Selection in the Construction of Multiply Disrupted Yeast Strains
- Construction and characterization of an SV40 mutant defective in nuclear transport of T antigen.
- Selection of multiple disruption events in Aspergillus fumigatus using the orotidine-5′-decarboxylase gene, pyrG, as a unique transformation marker
- Functional Reconstitution of a Fungal Natural Product Gene Cluster by Advanced Genome Editing.
Cited by
- Nonproteinaceous effectors: the terra incognita of plant-fungal interactions.
- Inducible Plasmid Self-Destruction (IPSD) assisted genome engineering in lactobacilli and bifidobacteria
- Genome editing in grass plants
- The Aspergillus fumigatus Phosphoproteome Reveals Roles of High-Osmolarity Glycerol Mitogen-Activated Protein Kinases in Promoting Cell Wall Damage and Caspofungin Tolerance
- CRISPR/Cas9 genome editing in ergot fungus Claviceps purpurea.
- Adapting CRISPR/Cas9 System for Targeting Mitochondrial Genome
- Current status and prospects of plant genome editing in Australia
- Mitochondria: Their relevance during oocyte ageing
- A Review of Recent Advances and Future Directions in the Management of Salinity Stress in Finger Millet
- Biosynthesis of Calipyridone A Represents a Fungal 2-Pyridone Formation without Ring Expansion in Aspergillus californicus.
- The Genetic Components of a Natural Color Palette: A Comprehensive List of Carotenoid Pathway Mutations in Plants
- Trichoderma – genomes and genomics as treasure troves for research towards biology, biotechnology and agriculture
- A Versatile in Vivo DNA Assembly Toolbox for Fungal Strain Engineering
- A Mad7 System for Genetic Engineering of Filamentous Fungi
- Gasdermin E as a potential target and biomarker for CRISPR-Cas9-based cancer therapy.
- Colistin enhances caspofungin antifungal efficacy against Aspergillus fumigatus by modulating calcium homeostasis and stress responses
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