Functional Reconstitution of a Fungal Natural Product Gene Cluster by Advanced Genome Editing.
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Summary
This work presents a CRISPR/Cas9-based tool for advanced molecular genetic studies in filamentous fungi, exploiting selectable markers separated from the edited locus by combining computational analysis with targeted gene editing.
- Type
- article
- Published
- 2017-01-20
- Cited by
- 74
- References
- 35
- OpenAlex
- https://openalex.org/W2514798302
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:206764597
Keywords
Biology, CRISPR, Genome editing, Polyketide synthase, Gene
References
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- Genome editing in Ustilago maydis using the CRISPR-Cas system.
- Synthetic biology of fungal natural products
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- How specific is CRISPR/Cas9 really?
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- A Point Mutation in ftmD Blocks the Fumitremorgin Biosynthetic Pathway in Aspergillus fumigatus Strain Af293
- Enzymatic assembly of DNA molecules up to several hundred kilobases
- AmcA—a putative mitochondrial ornithine transporter supporting fungal siderophore biosynthesis
- Human and Plant Fungal Pathogens: The Role of Secondary Metabolites
- Regulation of fungal secondary metabolism
- Trypacidin, a Spore-Borne Toxin from Aspergillus fumigatus, Is Cytotoxic to Lung Cells
- Microbial communication leading to the activation of silent fungal secondary metabolite gene clusters
- Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors.
- Upgrading Fungal Gene Expression on Demand: Improved Systems for Doxycycline-Dependent Silencing in Aspergillus fumigatus
- Development of a homologous transformation system for the human pathogenic fungus Aspergillus fumigatus based on the pyrG gene encoding orotidine 5′′-monophosphate decarboxylase
- Genetic surgery in fungi: employing site-specific recombinases for genome manipulation
Cited by
- Establishment of CRISPR/Cas9 in Alternaria alternata.
- Breaking the silence: new strategies for discovering novel natural products.
- Future insights in fungal metabolic engineering.
- Characterization of a major facilitator superfamily transporter in Shiraia bambusicola.
- CRISPR system in filamentous fungi: Current achievements and future directions.
- Genome editing in Shiraia bambusicola using CRISPR-Cas9 system.
- CRISPR/Cas9-based genome editing of the filamentous fungi: the state of the art
- Recent development of antiSMASH and other computational approaches to mine secondary metabolite biosynthetic gene clusters
- A Simple and Universal System for Gene Manipulation in Aspergillus fumigatus: In Vitro-Assembled Cas9-Guide RNA Ribonucleoproteins Coupled with Microhomology Repair Templates
- CRISPR-Cas9 mediated gene deletions in lager yeast Saccharomyces pastorianus
- HEx: A heterologous expression platform for the discovery of fungal natural products
- From genomics to metabolomics, moving toward an integrated strategy for the discovery of fungal secondary metabolites.
- Genetic engineering of host organisms for pharmaceutical synthesis.
- Recent Advances in Activating Silent Biosynthetic Gene Clusters in Bacteria
- Genome Editing: CRISPR-Cas9.
- Design of gRNA Expression Systems and Characterization of CRISPR Transcriptional Networks in Saccharomyces cerevisiae
- Strategies for Engineering Natural Product Biosynthesis in Fungi.
- Forced Recycling of an AMA1-Based Genome-Editing Plasmid Allows for Efficient Multiple Gene Deletion/Integration in the Industrial Filamentous Fungus Aspergillus oryzae
- CRISPR/Cas-based genome engineering in natural product discovery.
- The CRISPR/Cas9 System for Targeted Genome Engineering in Free-Living Fungi: Advances and Opportunities for Lichenized Fungi
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