CRISPR-Cas systems for genome editing, regulation and targeting
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Summary
A modified version of the CRISPR-Cas9 system has been developed to recruit heterologous domains that can regulate endogenous gene expression or label specific genomic loci in living cells, which will undoubtedly transform biological research and spur the development of novel molecular therapeutics for human disease.
- Type
- review
- Published
- 2014-03-02
- Cited by
- 3,002
- References
- 105
- Access
- Open access
- OpenAlex
- https://openalex.org/W2039705224
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:910259
Keywords
CRISPR, Genome editing, Cas9, Biology, Computational biology
References
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- RNA-guided genome editing in plants using a CRISPR-Cas system.
- Synergistic and tunable human gene activation by combinations of synthetic transcription factors
- Harnessing the CRISPR/Cas9 system to disrupt latent HIV-1 provirus
- Rapid “open-source” engineering of customized zinc-finger nucleases for highly efficient gene modification
- Precise and Heritable Genome Editing in Evolutionarily Diverse Nematodes Using TALENs and CRISPR/Cas9 to Engineer Insertions and Deletions
- Genome-Scale CRISPR-Cas9 Knockout Screening in Human Cells
- Efficient In Vivo Genome Editing Using RNA-Guided Nucleases
- Genome engineering using the CRISPR-Cas9 system
- Targeted DNA Demethylation and Endogenous Gene Activation Using Programmable TALE-TET1 Fusions
- Genome editing with engineered zinc finger nucleases
- Targeted mutagenesis in the model plant Nicotiana benthamiana using Cas9 RNA-guided endonuclease
- Standardized reagents and protocols for engineering zinc finger nucleases by modular assembly
- Chromosomal deletions and inversions mediated by TALENs and CRISPR/Cas in zebrafish
- Memory of viral infections by CRISPR-Cas adaptive immune systems: acquisition of new information.
Cited by
- Host genetic studies in adult pulmonary tuberculosis.
- Beyond traditional pharmacology: new tools and approaches
- Recombinant messenger RNA technology and its application in cancer immunotherapy, transcript replacement therapies, pluripotent stem cell induction, and beyond
- The Development of TALE Nucleases for Biotechnology
- Connexin-Based Therapeutics and Tissue Engineering Approaches to the Amelioration of Chronic Pancreatitis and Type I Diabetes: Construction and Characterization of a Novel Prevascularized Bioartificial Pancreas
- Stem Cells for Modeling and Therapy of Parkinson's Disease
- Using hiCLIP to identify RNA duplexes that interact with a specific RNA-binding protein
- A novel genetic tool for metabolic optimization of Corynebacterium glutamicum: efficient and repetitive chromosomal integration of synthetic promoter-driven expression libraries
- Using Morpholinos to Probe Gene Networks in Sea Urchin.
- A CRISPR New World: Attitudes in the Public toward Innovations in Human Genetic Modification
- Next-generation mammalian genetics toward organism-level systems biology
- Genome Editing of Food-Grade Lactobacilli To Develop Therapeutic Probiotics
- CRISPR/Cas9 Technology: Applications and Human Disease Modeling.
- Next-Generation Sequencing of Genome-Wide CRISPR Screens
- User-Friendly Genetic Conditional Knockout Strategies by CRISPR/Cas9
- CRISPR/Cas9 gene editing for the creation of an MGAT1-deficient CHO cell line to control HIV-1 vaccine glycosylation
- A limited number of double-strand DNA breaks is sufficient to delay cell cycle progression
- CRISPR–Cas9 in genome editing: Its function and medical applications
- Towards understanding the biosynthetic pathway for ustilaginoidin mycotoxins in Ustilaginoidea virens.
- CRISPR/Cas9-mediated genome editing of splicing mutation causing congenital hearing loss.
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