How specific is CRISPR/Cas9 really?
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Summary
This review will focus on deciphering and interpreting seemingly opposing claims about the specificity of CRISPR/Cas9 and its role in genome-editing.
- Type
- review
- Published
- 2015-10-24
- Cited by
- 116
- References
- 45
- Access
- Open access
- OpenAlex
- https://openalex.org/W1788559458
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:206496743
Keywords
CRISPR, Genome editing, Cas9, Computational biology, Biology
References
- Genome-wide binding of the CRISPR endonuclease Cas9 in mammalian cells
- Protospacer Adjacent Motif (PAM)-Distal Sequences Engage CRISPR Cas9 DNA Target Cleavage
- Dimeric CRISPR RNA-Guided FokI-dCas9 Nucleases Directed by Truncated gRNAs for Highly Specific Genome Editing
- Sequence determinants of improved CRISPR sgRNA design
- Rational design of highly active sgRNAs for CRISPR-Cas9-mediated gene inactivation
- DNA interrogation by the CRISPR RNA-guided endonuclease Cas9
- Low incidence of off-target mutations in individual CRISPR-Cas9 and TALEN targeted human stem cell clones detected by whole-genome sequencing
- Recombination initiation maps of individual human genomes
- Genome-wide detection of DNA double-stranded breaks induced by engineered nucleases
- GUIDE-Seq enables genome-wide profiling of off-target cleavage by CRISPR-Cas nucleases
- CAS9 transcriptional activators for target specificity screening and paired nickases for cooperative genome engineering
- Whole-Genome Sequencing Analysis Reveals High Specificity of CRISPR/Cas9 and TALEN-Based Genome Editing in Human iPSCs
- Genome-wide analysis reveals characteristics of off-target sites bound by the Cas9 endonuclease
- DNA targeting specificity of RNA-guided Cas9 nucleases
- Target specificity of the CRISPR-Cas9 system
- A Programmable Dual-RNA–Guided DNA Endonuclease in Adaptive Bacterial Immunity
- Unbiased detection of off-target cleavage by CRISPR-Cas9 and TALENs using integrase-defective lentiviral vectors
- Functional annotation of native enhancers with a Cas9 -histone demethylase fusion
- RNA-guided gene activation by CRISPR-Cas9-based transcription factors
- DNA Recombination
Cited by
- mCAL: A New Approach for Versatile Multiplex Action of Cas9 Using One sgRNA and Loci Flanked by a Programmed Target Sequence
- CRISPR/Cas9 in Genome Editing and Beyond
- Stepping toward therapeutic CRISPR
- Epigenome Editing: State of the Art, Concepts, and Perspectives.
- Genome Engineering with TALE and CRISPR Systems in Neuroscience
- Real-time observation of DNA recognition and rejection by the RNA-guided endonuclease Cas9
- Defining and improving the genome-wide specificities of CRISPR-Cas9 nucleases
- The role of Pygo2 during intestinal tumor initiation and progression in vivo
- CRISPR/Cas9: a breakthrough in generating mouse models for endocrinologists.
- Optimizing sgRNA position markedly improves the efficiency of CRISPR/dCas9-mediated transcriptional repression
- Versatility of chemically synthesized guide RNAs for CRISPR-Cas9 genome editing.
- The use of CRISPR/Cas associated technologies for cell transplant applications
- Functional Reconstitution of a Fungal Natural Product Gene Cluster by Advanced Genome Editing.
- Genome-Wide Assessment of Efficiency and Specificity in CRISPR/Cas9 Mediated Multiple Site Targeting in Arabidopsis
- Highly multiplexed CRISPR‐Cas9‐nuclease and Cas9‐nickase vectors for inactivation of hepatitis B virus
- The potential and pitfalls of de‐extinction
- Achieving Plant CRISPR Targeting that Limits Off‐Target Effects
- Diving into marine genomics with CRISPR/Cas9 systems.
- Genome editing: the road of CRISPR/Cas9 from bench to clinic
- Discovery of rice essential genes by characterizing a CRISPR‐edited mutation of closely related rice MAP kinase genes
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