Multiplexed Protein Quantitation in Saccharomyces cerevisiae Using Amine-reactive Isobaric Tagging Reagents*S
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Summary
It is found that inactivation of Upf1p and Xrn1p causes common as well as unique effects on protein expression, and the use of 4-fold multiplexing to enable relative protein measurements simultaneously with determination of absolute levels of a target protein using synthetic isobaric peptide standards.
- Type
- article
- Published
- 2004-12-01
- Cited by
- 4,266
- References
- 37
- Access
- Open access
- OpenAlex
- https://openalex.org/W2120016245
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:1979097
Keywords
Isobaric labeling, Chemistry, Isobaric process, Quantitative proteomics, Peptide
References
- Selective incorporation of isotopically labeled amino acids for identification of intact proteins on a proteome-wide level.
- Purification and characterization of yeast RNA polymerase II general initiation factor g.
- Fragments of the internal transcribed spacer 1 of pre-rRNA accumulate in Saccharomyces cerevisiae lacking 5'----3' exoribonuclease 1
- Quantitative profiling of differentiation-induced microsomal proteins using isotope-coded affinity tags and mass spectrometry
- SAM2 encodes the second methionine S-adenosyl transferase in Saccharomyces cerevisiae: physiology and regulation of both enzymes
- REB1, a yeast DNA-binding protein with many targets, is essential for growth and bears some resemblance to the oncogene myb
- Sec24p and Iss1p function interchangeably in transport vesicle formation from the endoplasmic reticulum in Saccharomyces cerevisiae.
- Probability-based protein identification by searching sequence databases using mass spectrometry data
- Depth of Proteome Issues
- A Novel Proteomic Approach for Specific Identification of Tyrosine Kinase Substrates Using [13C]Tyrosine*
- Complementary Profiling of Gene Expression at the Transcriptome and Proteome Levels in Saccharomyces cerevisiae*S
- A perfect message: RNA surveillance and nonsense-mediated decay.
- A yeast gene important for protein assembly into the endoplasmic reticulum and the nucleus has homology to DnaJ, an Escherichia coli heat shock protein
- Genome-wide analysis of mRNAs regulated by the nonsense-mediated and 5' to 3' mRNA decay pathways in yeast.
- Stable isotope-coded proteomic mass spectrometry.
- Nonsense-mediated mRNA decay in Saccharomyces cerevisiae.
- Advances in quantitative proteomics via stable isotope tagging and mass spectrometry.
- Tandem mass tags: a novel quantification strategy for comparative analysis of complex protein mixtures by MS/MS.
- Properties of 13C-substituted arginine in stable isotope labeling by amino acids in cell culture (SILAC).
- Reproducibility of quantitative proteomic analyses of complex biological mixtures by multidimensional protein identification technology.
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- The heat shock response of Synechocystis sp. PCC 6803 analysed by transcriptomics and proteomics.
- The Association of Biomolecular Resource Facilities Proteomics Research Group 2006 Study
- Multidimensional liquid phase protein separations in conjunction with stable isotope labelling for quantitative proteomics
- New ways in qualitative and quantitative protein analysis: nano chromatography coupled to element mass spectrometry.
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- Investigation of Energy Transfer, Quantification, and Localization of Peptides and Proteins by Fluorescence Spectroscopy and Mass Spectrometry
- New dimensions in the study of protein complexes using quantitative mass spectrometry
- Bioanalysis of recombinant proteins and antibodies by mass spectrometry.
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