A yeast gene important for protein assembly into the endoplasmic reticulum and the nucleus has homology to DnaJ, an Escherichia coli heat shock protein
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Summary
Conditional- lethal mutants (npl, for nuclear protein localization) that missorted NLS-cytochrome c1 to the mitochondria, allowing growth on glycerol are isolated and may act at a very early common step in localization of proteins to the nucleus and the ER.
- Type
- article
- Published
- 1989-12-01
- Cited by
- 272
- References
- 45
- Access
- Open access
- OpenAlex
- https://openalex.org/W2022936503
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:1246216
Keywords
Biology, Endoplasmic reticulum, Nuclear localization sequence, Cell biology, Inner membrane
References
- Methods in yeast genetics
- Import of proteins into mitochondria. The precursor of cytochrome c1 is processed in two steps, one of them heme-dependent.
- Nucleotide sequence of the Escherichia coli dnaJ gene and purification of the gene product.
- High‐affinity binding sites involved in the import of porin into mitochondria.
- The yeast regulatory gene PHO2 encodes a homeo box.
- 70K heat shock related proteins stimulate protein translocation into microsomes
- Homologous plant and bacterial proteins chaperone oligomeric protein assembly
- Amino terminus of the yeast GAL4 gene product is sufficient for nuclear localization.
- A subfamily of stress proteins facilitates translocation of secretory and mitochondrial precursor polypeptides
- Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors.
- A short amino acid sequence able to specify nuclear location.
- Speculations on the functions of the major heat shock and glucose-regulated proteins.
- Calcium-dependent bacteriophage DNA infection.
- Purification of a membrane-associated protein complex required for protein translocation across the endoplasmic reticulum.
- Specialized nucleoprotein structures at the origin of replication of bacteriophage lambda: localized unwinding of duplex DNA by a six-protein reaction.
- Sequence requirements for nuclear location of simian virus 40 large-T antigen
- Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencing.
- Isolation of genes by complementation in yeast: molecular cloning of a cell-cycle gene.
- Two nuclear mutations that block mitochondrial protein import in yeast.
- Mutations that alter both localization and production of a yeast nuclear protein.
Cited by
- Do cytosolic factors prevent promiscuity at the membrane surface?
- Roles for hsp70 in protein translocation across membranes of organelles.
- Isolation and sequence of HSP30, a yeast heat-shock gene coding for a hydrophobic membrane protein
- Sls1p Stimulates Sec63p-Mediated Activation of Kar2p in a Conformation-Dependent Manner in the Yeast Endoplasmic Reticulum
- Nuclear Export of hnRNP Hrp1p and Nuclear Export of hnRNP Npl3p Are Linked and Influenced by the Methylation State of Npl3p
- Cloning and characterisation of SSV genes from Saccharomyces Cerevisiae
- Fifty years of nuclear pores and nucleocytoplasmic transport studies: multiple tools revealing complex rules.
- Components and mechanisms involved in transport of proteins into the endoplasmic reticulum.
- Normal protein folding machinery.
- A second trimeric complex containing homologs of the Sec61p complex functions in protein transport across the ER membrane of S. cerevisiae.
- Cloning and characterization of heat shock protein DnaJ homologues from Plasmodium falciparum and comparison with ring infected erythrocyte surface antigen1Note: Nucleotide sequence data reported in this paper is available in the DDJB data bases under the accession number D85686.1
- Secretory pathway function in Saccharomyces cerevisiae.
- The fate of endoplasmic reticulum-targeted proteins in the face of proteasome failure
- Protein Synthesis and Targeting in Yeast
- Structure and mechanism of 70-kDa heat-shock-related proteins.
- The mitochondrial protein targeting suppressor (mts1) mutation maps to the mRNA‐binding domain of Npl3p and affects translation on cytoplasmic polysomes
- Molecular chaperones and intracellular protein translocation.
- Two birds with one stone: genes that encode products targeted to two or more compartments
- Zum Mechanismus der Translokation von Proteinen in das Endoplasmatische Retikulum der Hefe
- Responses of an Environmental Gram-negative Bacterium to Pollutant Stress
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