Fragments of the internal transcribed spacer 1 of pre-rRNA accumulate in Saccharomyces cerevisiae lacking 5'----3' exoribonuclease 1
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Summary
A portion of the internal transcribed spacer 1 found on 20S pre-rRNA accumulates in Saccharomyces cerevisiae lacking 5'----3' exoribonuclease 1, showing that an endonucleolytic cleavage at the 3' terminus of 18S rRNA is involved in the 20SPre-r RNA to 18S mature rRNA conversion.
- Type
- article
- Published
- 1991-11-01
- Cited by
- 118
- References
- 19
- Access
- Open access
- OpenAlex
- https://openalex.org/W1673502203
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:19280097
Keywords
Exoribonuclease, Internal transcribed spacer, Biology, Saccharomyces cerevisiae, Spacer DNA
References
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- Methods in yeast genetics
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- A 5'----3' exoribonuclease of Saccharomyces cerevisiae: size and novel substrate specificity.
- Disruption of the gene XRN1, coding for a 5'----3' exoribonuclease, restricts yeast cell growth.
- Lariat structures are in vivo intermediates in yeast pre-mRNA splicing.
- Purification and characterization of an activity from Saccharomyces cerevisiae that catalyzes homologous pairing and strand exchange.
- Labeling of RNA and phosphoproteins in Saccharomyces cerevisiae.
- Some characteristics of processing sites in ribosomal precursor RNA of yeast
- Terminal nucleotide sequences of 17-S ribosomal RNA and its immediate precursor 18-S RNA in yeast.
- Isolation and properties of a single-strand 5'----3' exoribonuclease from Ehrlich ascites tumor cell nucleoli.
- Accurate processing of human pre-rRNA in vitro
- Genomic Sequencing
Cited by
- Saccharomyces cerevisiae RAI1 (YGL246c) Is Homologous to Human DOM3Z and Encodes a Protein That Binds the Nuclear Exoribonuclease Rat1p
- Exoribonucleases and their multiple roles in RNA metabolism.
- Analysis of the in vivo functions and assembly pathway of small subunit ribosomal proteins in Saccharomyces cerevisiae
- Two distinct recognition signals define the site of endonucleolytic cleavage at the 5′‐end of yeast 18S rRNA.
- Overview of Saccharomyces cerevisiae ribonucleases.
- 5'-exoribonuclease 1: Xrn1.
- Requirements for the nuclear export of the small ribosomal subunit.
- In vitro assays of 5' to 3'-exoribonuclease activity.
- Processing of mammalian rRNA precursors at the 3' end of 18S rRNA. Identification of cis-acting signals suggests the involvement of U13 small nucleolar RNA.
- Analysis of XRN orthologs by complementation of yeast mutants and localization of XRN-GFP fusion proteins.
- Processing of eukaryotic ribosomal RNA.
- Processing of the Intron-Encoded U18 Small Nucleolar RNA in the Yeast Saccharomyces cerevisiaeRelies on Both Exo- and Endonucleolytic Activities
- Nip7p Interacts with Nop8p, an Essential Nucleolar Protein Required for 60S Ribosome Biogenesis, and the Exosome Subunit Rrp43p
- NDT80, a meiosis-specific gene required for exit from pachytene in Saccharomyces cerevisiae
- Function and synthesis of small nucleolar RNAs.
- 5′-Exonuclease-2 of Saccharomyces cerevisiae
- Characterization of the XRN1 gene encoding a 5'-->3' exoribonuclease: sequence data and analysis of disparate protein and mRNA levels of gene-disrupted yeast cells.
- Analysis of two domains with novel RNA-processing activities throws light on the complex evolution of ribosomal RNA biogenesis
- Cloning and characterization of mouse Dhm2 cDNA, a functional homolog of budding yeast SEP1.
- Yeast ribosomal proteins L4, L17, L20, and L25 exhibit different binding characteristics for the yeast 35S precursor rRNA.
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