A new mathematical model for relative quantification in real-time RT-PCR.
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Summary
This study enters into the particular topics of the relative quantification in real-time RT-PCR of a target gene transcript in comparison to a reference gene transcript and presents a new mathematical model that needs no calibration curve.
- Type
- article
- Published
- 2001-05-01
- Cited by
- 34,028
- References
- 26
- Access
- Open access
- OpenAlex
- https://openalex.org/W2108244474
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:9003591
Keywords
Biology, Real-time polymerase chain reaction, Computational biology, Polymerase chain reaction, Complementary DNA
References
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- Quantification of insulin-like growth factor-1 (IGF-1) mRNA: Development and validation of an internally standardised competitive reverse transcription-polymerase chain reaction
- Quantification of murine cytokine mRNAs using real time quantitative reverse transcriptase PCR.
- Quantitative RT-PCR: limits and accuracy.
- Quantification of low-copy transcripts by continuous SYBR Green I monitoring during amplification.
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- Molecular Cloning: A Laboratory Manual
- A novel method for real time quantitative RT-PCR.
Cited by
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- Mécanismes moléculaires impliqués dans la modulation de la production de trichothécènes de type B par Fusarium graminearum en réponse au stress oxydant
- Biodegradation of a sulfur-containing PAH, dibenzothiophene, by a mixed bacterial community
- Gene expression analysis by quantitative real-time PCR for floral tissues.
- Development of improved diagnostic testing methods for invasive pneumococcal disease
- Effetto della disfunzione epatica sull’induzione enzimatica: valutazione dell’inducibilità dei CYP1A e CYP3A in ratti con cirrosi sperimentalmente indotta
- Use of self-quenched, fluorogenic LUX primers for gene expression profiling.
- Propagation and modulation of activity in early olfactory processing and its relevance to odor-driven behavior
- Molecular characterization and functional analysis of CzR1, a coiled-coil-nucleotide-binding-site-leucine-rich repeat R-gene from Curcuma zedoaria Loeb. that confers resistance to Pythium aphanidermatum
- Salt stress responses in pear and quince: physiological and molecular aspects
- Assessing oxygen sensitivity of the multidrug resistance (MDR) gene.
- Stress, mixed messages, and hormone signaling : regulation of translation and the unfolded protein response in pituitary gonadotropes
- GABA y receptores GABA B en el eje reproductivo: su relación con GnRH y kisspeptinas
- Characterization of two PR genes from Fragaria chiloensis in response to Botrytis cinerea infection: A comparison with Fragaria x ananassa
- Metabolic and Transcriptomic Responses of Weaned Pigs Induced by Different Dietary Amylose and Amylopectin Ratio
- The spectrum of cytokeratins expressed in the adult human cornea, limbus and perilimbal conjunctiva.
- Statistical Analysis of Quantitative RT-PCR Results.
- Vitreous induces components of the prostaglandin E2 pathway in human retinal pigment epithelial cells.
- Ca2+-dependent Regulation of TrkB Expression in Neurons*
- Repeated stress in combination with pyridostigmine Part II: changes in cerebral gene expression.
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