Quantification of insulin-like growth factor-1 (IGF-1) mRNA: Development and validation of an internally standardised competitive reverse transcription-polymerase chain reaction
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Summary
The present method was first applied to study the effect of long term castration on the IGF-1 expression rates in bovine tissues, and the hepatic IGF- 1 mRNA concentrations were well correlated with the plasma concentrations as quantified by RIA and were higher in intact than in castrated animals.
- Type
- article
- Published
- 2009-07-14
- Cited by
- 45
- References
- 28
- OpenAlex
- https://openalex.org/W2089108304
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:1806330
Keywords
Messenger RNA, Primer (cosmetics), Reverse transcription polymerase chain reaction, Molecular biology, Reverse transcriptase
References
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- Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase.
- Nucleotide sequence of the bovine insulin-like growth factor 1 (IGF-1) and its IGF-1A precursor.
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- Quantification of androgen receptor mRNA in tissues by competitive co-amplification of a template in reverse transcription-polymerase chain reaction.
- Rapid separation, quantitation and purification of products of polymerase chain reaction by liquid chromatography.
- Absolute mRNA quantification using the polymerase chain reaction (PCR). A novel approach by a PCR aided transcript titration assay (PATTY).
- Quantitation of mRNA by the polymerase chain reaction.
- Insulin-like growth factors and their binding proteins.
- Studies on monozygous cattle twins. 23. The effect of castration and age of castration on the development of single muscles, bones and special sex characters. Part 2.
- Androgen and estrogen receptors in bovine skeletal muscle: relation to steroid-induced allometric muscle growth.
- Muscle and liver cell growth: role of hormones and nutritional factors.
Cited by
- Insulinartige Wachstumsfaktor-1- (IGF-1-)mRNA und IGF-1-Protein Expression in den Zellen des Trabekelwerks im Rinderauge
- Effect of testosterone on insulin-like growth factor-I, androgen receptor, and myostatin gene expression in splenius and semitendinosus muscles in sheep.
- Genomic organization, promoter characterization and expression analysis of the leukocyte cell-derived chemotaxin-2 gene in Epinephelus akaraa.
- Absolute quantitation of RNA by a competitive real-time RT-PCR method using piscine nodavirus as a model.
- Intestinal expression of metal transporters in Wilson’s disease
- Long-term effects of growth hormone replacement therapy on liver function in adult patients with growth hormone deficiency.
- mRNA of insulin-like growth factor (IGF) quantification and presence of IGF binding proteins, and receptors for growth hormone, IGF-I and insulin, determined by reverse transcribed polymerase chain reaction, in the liver of growing and mature male cattle.
- Long-term effects of mycophenolic acid on the immunoglobulin and inflammatory marker-gene expression in sheep white blood cells
- A quantitative reverse transcription and polymerase chain reaction assay for human IGF-II allows direct comparison of IGF-II mRNA levels in cancerous breast, bladder, and prostate tissues.
- Insulin-like growth factor-1 gene cloning and protein expression in bovine trabecular meshwork tissue and cells
- Phosphocitrate Is Potentially a Disease-Modifying Drug for Noncrystal-Associated Osteoarthritis
- qPCR, dPCR, NGS – A journey
- Insulin increases the activity of mesangial BK channels through MAPK signaling.
- Relative expression software tool (REST©) for group-wise comparison and statistical analysis of relative expression results in real-time PCR
- The response of the hepatic insulin-like growth factor system to growth hormone and dexamethasone in calves.
- Ethylene-binding activity, gene expression levels, and receptor system output for ethylene receptor family members from Arabidopsis and tomato.
- A new mathematical model for relative quantification in real-time RT-PCR.
- Validities of mRNA quantification using recombinant RNA and recombinant DNA external calibration curves in real-time RT-PCR
- Microarray-Based Gene Expression Profiling to Elucidate Effectiveness of Fermented Codonopsis lanceolata in Mice
- Effects of synthetic progestagens on the mRNA expression of androgen receptor, progesterone receptor, oestrogen receptor alpha and beta, insulin-like growth factor-1 (IGF-1) and IGF-1 receptor in heifer tissues.
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