Kinetic Outlier Detection (KOD) in real-time PCR.
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Summary
Kinetic Outlier Detection, a statistical method based on a comparison of PCR efficiency, estimated from the amplification curve of a test sample, with the mean PCR efficiency of samples in a training set, may be a useful tool for outlier detection in real-time PCR.
- Type
- article
- Published
- 2003-09-01
- Cited by
- 114
- References
- 40
- Access
- Open access
- OpenAlex
- https://openalex.org/W2100122767
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:9913676
Keywords
Biology, Outlier, Real-time polymerase chain reaction, Similarity (geometry), Nucleic acid
References
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- Reverse Transcriptase (RT) Inhibition of PCR at Low Concentrations of Template and Its Implications for Quantitative RT-PCR
- The presence of a pseudogene may affect the use of HPRT as an endogenous mRNA control in RT-PCR.
- Inhibition of PCR by components of food samples, microbial diagnostic assays and DNA-extraction solutions.
- Counting target molecules by exponential polymerase chain reaction: copy number of mitochondrial DNA in rat tissues.
- Control genes in reverse transcriptase-polymerase chain reaction assays
- Housekeeping genes as internal standards: use and limits.
- Use of the recursion formula of the Gompertz function for the quantitation of PCR-amplified templates.
- Reliability of reverse transcription-polymerase chain reaction (RT-PCR)-based assays for the detection of circulating tumour cells: a quality-assurance initiative of the EORTC Melanoma Cooperative Group.
- Light-up-probe-based real-time Q-PCR
- Purification and Characterization of PCR-Inhibitory Components in Blood Cells
- Quantitative Real-Time PCR Method for Detection of B-Lymphocyte Monoclonality by Comparison of κ and λ Immunoglobulin Light Chain Expression
- SoFAR: software for fully automatic evaluation of real-time PCR data.
- A new mathematical model for relative quantification in real-time RT-PCR.
- Standardization and Quality Control of PCR Analyses
- Validities of mRNA quantification using recombinant RNA and recombinant DNA external calibration curves in real-time RT-PCR
- A novel method to compensate for different amplification efficiencies between patient DNA samples in quantitative real-time PCR.
Cited by
- An inter-platform repeatability study investigating real-time amplification of plasmid DNA
- Kinetics quality assessment for relative quantification by real-time PCR.
- Microarray analysis of Acidovorax temperans
- Studies of Innate and Adaptive Immunity in Islet Transplantation
- Identificación y cuantificación de especies del género Merluccius mediante la utilización de PCR a tiempo real
- A standard curve based method for relative real time PCR data processing
- Comparison of Analytic Methods for Quantitative Real-Time Polymerase Chain Reaction Data
- Family study of high myopia : association studies
- An improvement of the 2ˆ(–delta delta CT) method for quantitative real-time polymerase chain reaction data analysis
- Target estimation in real-time polymerase chain reaction using sequential Monte Carlo
- Diversity and Distribution of Marine Synechococcus: Multiple Gene Phylogenies for Consensus Classification and Development of qPCR Assays for Sensitive Measurement of Clades in the Ocean
- Comprehensive Algorithm for Quantitative Real-Time Polymerase Chain Reaction
- Establishment of TaqMan Real-time Quantitative PCR Assay for Foreign Gene Copy Numbers in Transgenic Soybean
- Shape based kinetic outlier detection in real-time PCR
- The effect of dilution and the use of a post-extraction nucleic acid purification column on the accuracy, precision, and inhibition of environmental DNA samples
- Salmonella enterica serovar Choleraesuis infection of the porcine jejunal Peyer's patch rapidly induces IL-1beta and IL-8 expression.
- Statistical algorithm for assuring similar efficiency in standards and samples for absolute quantification by real-time reverse transcription polymerase chain reaction.
- Analysis of biological and technical variability in gene expression assays from formalin-fixed paraffin-embedded classical Hodgkin lymphomas.
- Quantitative real-time PCR for cancer detection: the lymphoma case
- Post‐transplant upregulation of chemokine messenger RNA in non‐human primate recipients of intraportal pig islet xenografts
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