An improvement of the 2ˆ(–delta delta CT) method for quantitative real-time polymerase chain reaction data analysis
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Summary
The improved method, the individual efficiency corrected calculation, produces more accurate estimates in relative gene expression than the 2-ΔΔCT method and is thus a better way to calculate relative gene expression.
- Type
- article
- Published
- 2013-08-01
- Cited by
- 1,822
- References
- 34
- Access
- Open access
- OpenAlex
- https://openalex.org/W1809721560
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:6315078
Keywords
Background subtraction, Real-time polymerase chain reaction, Subtraction, Polymerase chain reaction, Biological system
References
- Prostanoid receptor gene expression profile in human trabecular meshwork: a quantitative real-time PCR approach.
- Assumption-free analysis of quantitative real-time polymerase chain reaction (PCR) data.
- Rapid Cycle Real-Time PCR — Methods and Applications
- [Real-time PCR: approaches to data analysis (a review)].
- Evaluation of internal control genes for qRT-PCR normalization in tissues and cell culture for antiviral studies of grass carp (Ctenopharyngodon idella).
- Comprehensive Algorithm for Quantitative Real-Time Polymerase Chain Reaction
- Development and validation of real-time quantitative reverse transcriptase-polymerase chain reaction for monitoring gene expression in cardiac myocytes in vitro.
- Utility of the Housekeeping Genes 18S rRNA, β‐Actin and Glyceraldehyde‐3‐Phosphate‐Dehydrogenase for Normalization in Real‐Time Quantitative Reverse Transcriptase‐Polymerase Chain Reaction Analysis of Gene Expression in Human T Lymphocytes
- Determining suitable internal standards for mRNA quantification of increasing cancer progression in human breast cells by real-time reverse transcriptase polymerase chain reaction.
- Avoiding Pitfalls of Internal Controls: Validation of Reference Genes for Analysis by qRT-PCR and Western Blot throughout Rat Retinal Development
- Revisiting the sigmoidal curve fitting applied to quantitative real-time PCR data.
- Validation of the 2-DeltaDeltaCt calculation as an alternate method of data analysis for quantitative PCR of BCR-ABL P210 transcripts.
- Standardized determination of real-time PCR efficiency from a single reaction set-up.
- Determination of stable housekeeping genes, differentially regulated target genes and sample integrity: BestKeeper – Excel-based tool using pair-wise correlations
- Kinetic Outlier Detection (KOD) in real-time PCR.
- A new real-time PCR method to overcome significant quantitative inaccuracy due to slight amplification inhibition
- Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method.
- Quantification of mRNA using real-time reverse transcription PCR (RT-PCR): trends and problems.
- Analyzing real-time PCR data by the comparative CT method
- A new quantitative method of real time reverse transcription polymerase chain reaction assay based on simulation of polymerase chain reaction kinetics.
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- Vitamin D restriction enhances periovarian adipose tissue inflammation in a model of menopause
- Has‑miR‑125a and 125b are induced by treatment with cisplatin in nasopharyngeal carcinoma and inhibit apoptosis in a p53‑dependent manner by targeting p53 mRNA.
- A regulatory circuit involving miR-143 and DNMT3a mediates vascular smooth muscle cell proliferation induced by homocysteine.
- Therapeutic effects of bone marrow-derived mesenchymal stem cells on radiation-induced lung injury.
- Morin suppresses inflammatory cytokine expression by downregulation of nuclear factor-κB and mitogen-activated protein kinase (MAPK) signaling pathways in lipopolysaccharide-stimulated primary bovine mammary epithelial cells.
- Culture & differentiation of mesenchymal stem cell into osteoblast on degradable biomedical composite scaffold: In vitro study
- Unaccounted uncertainty from qPCR efficiency estimates entails uncontrolled false positive rates
- Analysis of Kynurenine/Tryptophan ratio and expression of IDO1 and 2 mRNA in tumour tissue of cervical cancer patients.
- Waxcrystal-sparse leaf 3 encoding a β-ketoacyl-CoA reductase is involved in cuticular wax biosynthesis in rice
- Environmental, Toxicological, and Evolutionary Influences on Membrane Composition in Fish
- Analysis of microarray-identified genes and microRNAs associated with drug resistance in ovarian cancer.
- NFAT5/STAT3 interaction mediates synergism of high salt with IL-17 towards induction of VEGF-A expression in breast cancer cells
- Silver nanoparticle toxicity is related to coating materials and disruption of sodium concentration regulation
- A practical qPCR approach to detect TERRA, the elusive telomeric repeat-containing RNA.
- Ureaplasma diversum Genome Provides New Insights about the Interaction of the Surface Molecules of This Bacterium with the Host
- Expression of Human Tissue Kallikreins (KLKs) in Polymorphous Low Grade Adenocarcinoma (PLGA)
- Overview of Genetic Background Beyond Polysaccharide Intercellular Adhesion Production in Staphylococcus epidermidis
- RNAi reveals proteins for metabolism and protein processing associated with Langat virus infection in Ixodes scapularis (black-legged tick) ISE6 cells
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