Accurate and Precise DNA Quantification in the Presence of Different Amplification Efficiencies Using an Improved Cy0 Method
Explore this paper's citation graph
Summary
The improved Cy0 method represents a simple yet powerful approach for reliable DNA quantification even in the presence of marked efficiency variations.
- Type
- article
- Published
- 2013-07-08
- Cited by
- 18
- References
- 37
- Access
- Open access
- OpenAlex
- https://openalex.org/W2073821470
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:9010701
Keywords
Biological system, Inflection point, Computer science, Computational biology, Materials science
References
- Assumption-free analysis of quantitative real-time polymerase chain reaction (PCR) data.
- Fine needle aspiration coupled with real-time PCR: a painless methodology to study adaptive functional changes in skeletal muscle.
- A standard curve based method for relative real time PCR data processing
- Large scale real-time PCR validation on gene expression measurements from two commercial long-oligonucleotide microarrays
- Evaluation of absolute quantitation by nonlinear regression in probe-based real-time PCR
- Identification of the heme compound copurified with deoxyribonucleic acid (DNA) from bloodstains, a major inhibitor of polymerase chain reaction (PCR) amplification.
- Inhibition of PCR by components of food samples, microbial diagnostic assays and DNA-extraction solutions.
- Shape based kinetic outlier detection in real-time PCR
- The expression analysis of mouse interleukin-6 splice variants argued against their biological relevance.
- Quantification of mRNA using real-time RT-PCR
- TATA binding protein associated factor 3 (TAF3) interacts with p53 and inhibits its function
- Twenty-five years of quantitative PCR for gene expression analysis.
- Validation of kinetics similarity in qPCR
- Assessing the Performance Capabilities of LRE-Based Assays for Absolute Quantitative Real-Time PCR
- Kinetic Outlier Detection (KOD) in real-time PCR.
- Design and optimization of reverse-transcription quantitative PCR experiments.
- A new real-time PCR method to overcome significant quantitative inaccuracy due to slight amplification inhibition
- The PCR plateau phase - towards an understanding of its limitations.
- A Flexible Growth Function for Empirical Use
- Inhibition and facilitation of nucleic acid amplification
Cited by
- Savitzky-Golay smoothing and differentiation for polymerase chain reaction quantification.
- A framework for analysis of real-time nucleic acid amplification data using novel multidimensional standard curves
- Transcriptome signature for dietary fructose-specific changes in rat renal cortex: A quantitative approach to physiological relevance
- Framework for DNA Quantification and Outlier Detection Using Multidimensional Standard Curves
- A novel data processing method CyC* for quantitative real time polymerase chain reaction minimizes cumulative error
- Kinetics of different bioreactor systems with Acidithiobacillus ferrooxidans for ferrous iron oxidation
- PANDAA-monium: Intentional violations of conventional qPCR design enables rapid, HIV-1 subtype-independent drug resistance SNP detection
- PANDAA intentionally violates conventional qPCR design to enable durable, mismatch-agnostic detection of highly polymorphic pathogens
- Genomic analysis of high copy-number sequences for the targeted detection of Listeria species using a flow-through surveillance system
- shinyCurves, a shiny web application to analyse multisource qPCR amplification data: a COVID-19 case study
- Robust and Precise Quantitative Real-Time Polymerase Chain Reaction with an Amplification Efficiency-Aware Reaction Kinetics Model.
- Clinical Validation of a Colorimetric Loop-Mediated Isothermal Amplification Using a Portable Device for the Rapid Detection of SARS-CoV-2
- Data treatment methods for real-time colorimetric loop-mediated isothermal amplification reactions
- Improved protocol for Bst polymerase and reverse transcriptase production and application to a point-of-care diagnostics system
- Automation and standardisation of a quantitative multiplex PCR assay using PCR.Ai.
- A survey of tools for the analysis of quantitative PCR (qPCR) data
- Analysis of TaqMan Array Cards Data by an Assumption-Free Improvement of the maxRatio Algorithm Is More Accurate than the Cycle-Threshold Method
- Additional file 2 of shinyCurves, a shiny web application to analyse multisource qPCR amplification data: a COVID-19 case study
- Draft 1 Savitzky-Golay smoothing and differentiation for PCR quantification 1
- A new diagnostic kit using real-time polymerase chain reaction for the detection of Mycobacterium tuberculosis