Quantification of mRNA using real-time RT-PCR
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Summary
A series of RT-qPCR protocols are described that illustrate the essential technical steps required to generate quantitative data that are reliable and reproducible in molecular medicine, biotechnology, microbiology and diagnostics.
- Type
- article
- Published
- 2006-11-01
- Cited by
- 2,287
- References
- 70
- OpenAlex
- https://openalex.org/W2008183439
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:10108148
Keywords
Computational biology, Snapshot (computer storage), Database normalization, Real-time polymerase chain reaction, Biology
References
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- Kinetic PCR Analysis: Real-time Monitoring of DNA Amplification Reactions
- Quantitative real-time PCR for cancer detection: the lymphoma case
- Simultaneous Amplification and Detection of Specific DNA Sequences
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- Evaluation of a 'one tube' reverse transcription-polymerase chain reaction for the detection of ruminant pestiviruses.
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Cited by
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- Gene expression analysis by quantitative real-time PCR for floral tissues.
- Salt stress responses in pear and quince: physiological and molecular aspects
- Differentially regulated genes in Solanum tuberosum in response to " Mexican potato purple top phytoplasma" infection
- Characterization and expression analyses of anti-apoptotic Bcl-2-like genes NR-13, Mcl-1, Bcl-X1, and Bcl-X2 in Atlantic cod (Gadus morhua).
- Defining cell populations with single-cell gene expression profiling: correlations and identification of astrocyte subpopulations
- Automatic genomics: a user-friendly program for the automatic designing and plate loading of medium-throughput qPCR experiments.
- Hypothyroidism in Adult Male Rats Alters Posttranscriptional Mechanisms of Luteinizing Hormone Biosynthesis
- Electroanalysis of single-nucleotide polymorphism by hairpin DNA architectures
- Ecological comparison of cellular stress responses among populations – normalizing RT-qPCR values to investigate differential environmental adaptations
- Assessment of reference gene stability influenced by extremely divergent disease symptoms in Solanum lycopersicum L.
- Validation of a real-time quantitative polymerase chain reaction method for the quantification of 3 survivin transcripts and evaluation in breast cancer tissues.
- Delayed protein shut down and cytopathic changes lead to high yields of infectious pancreatic necrosis virus cultured in Asian Grouper cells.
- Consensus policies to solve bioinformatic problems through Bayesian network classifiers and estimation of distribution algorithms
- Selection of Reference Genes for Expression Analysis in Diuraphis noxia (Hemiptera: Aphididae) Fed on Resistant and Susceptible Wheat Plants
- Maternal overnutrition enhances mRNA expression of adipogenic markers and collagen deposition in skeletal muscle of beef cattle fetuses.
- PRENATAL ALCOHOL EXPOSURE ALTERS STEADY-STATE AND ACTIVATED GENE EXPRESSION IN THE ADULT RAT BRAIN
- A Bead-Based Microfluidic Approach to Integrated Single-Cell Gene Expression Analysis by Quantitative RT-PCR
- Using quantitative PCR with retrotransposon-based insertion polymorphisms as markers in sugarcane
- Selection and Evaluation of Tissue Specific Reference Genes in Lucilia sericata during an Immune Challenge
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