Fine needle aspiration coupled with real-time PCR: a painless methodology to study adaptive functional changes in skeletal muscle.
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Summary
This painless methodology can be used to investigate, in vivo, human muscle RNA and DNA adaptations in response to either physiological and/or pharmacological stimuli.
- Type
- article
- Published
- 2007-06-01
- Cited by
- 25
- References
- 37
- OpenAlex
- https://openalex.org/W2077929470
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:1679761
Keywords
Skeletal muscle, Vastus lateralis muscle, Biology, Muscle biopsy, Real-time polymerase chain reaction
References
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- Multiplexed tandem PCR: gene profiling from small amounts of RNA using SYBR Green detection
- Quantification of multiple gene expression in individual cells.
- Mitochondrial oxidative function in human saponin‐skinned muscle fibres: effects of prolonged exercise
- Coordinated reduction of genes of oxidative metabolism in humans with insulin resistance and diabetes: Potential role of PGC1 and NRF1
- Mitochondrial DNA Deletion Mutations and Sarcopenia
- Single-cell RT-PCR as a tool to study gene expression in central and peripheral autonomic neurones.
- Effects of aerobic training on gene expression in skeletal muscle of elderly men.
- Mitochondrial DNA mutations as a fundamental mechanism in physiological declines associated with aging
- Influences of endurance training on the ultrastructural composition of the different muscle fiber types in humans
- Peroxisome proliferator-activated receptor-gamma coactivator 1 alpha (PGC-1 alpha): transcriptional coactivator and metabolic regulator.
- Real-time PCR in virology.
- Exercise induces transient transcriptional activation of the PGC‐1α gene in human skeletal muscle
- A new mathematical model for relative quantification in real-time RT-PCR.
- Plasticity of skeletal muscle mitochondria: structure and function.
Cited by
- Gene doping: possibilities and practicalities.
- Tiny percutaneous needle biopsy: An efficient method for studying cellular and molecular aspects of skeletal muscle in humans.
- Gene doping: the hype and the reality
- Two weekly sessions of combined aerobic and resistance exercise are sufficient to provide beneficial effects in subjects with Type 2 diabetes mellitus and metabolic syndrome
- Dietary fat differentially modulate the mRNA expression levels of oxidative mitochondrial genes in skeletal muscle of healthy subjects.
- Similar changes of gene expression in human skeletal muscle after resistance exercise and multiple fine needle biopsies.
- The Pleiotropic Effect of Physical Exercise on Mitochondrial Dynamics in Aging Skeletal Muscle
- Accurate and Precise DNA Quantification in the Presence of Different Amplification Efficiencies Using an Improved Cy0 Method
- The hunt for gene dopers.
- Mitohormesis in muscle cells: a morphological, molecular, and proteomic approach.
- Physical activity for the treatment and prevention of metabolic syndrome
- Quantitative evaluation of the mitochondrial DNA depletion syndrome.
- Latissimus dorsi fine needle muscle biopsy: a novel and efficient approach to study proximal muscles of upper limbs.
- biopsiesafter resistance exercise and multiple fine needle Similar changes of gene expression in human skeletal
- Genetic doping: WADA we do about the future of ‘cheating’ in sport?
- Muscle and Systemic Molecular Responses to a Single Flywheel Based Iso-Inertial Training Session in Resistance-Trained Men
- Myosin Heavy Chain Composition, Creatine Analogues, and the Relationship of Muscle Creatine Content and Fast-Twitch Proportion to Wilks Coefficient in Powerlifters.
- Altered muscle mitochondrial, inflammatory and trophic markers, and reduced exercise training adaptations in type 1 diabetes
- The skeletal muscle microbiopsy method in exercise and sports science research: A narrative and methodological review
- Skeletal Muscle Microbiopsies in Children and Adults—Tolerability, Sample Yield, and Analyzability
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