RNA-Guided Human Genome Engineering via Cas9
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Summary
The type II bacterial CRISPR system is engineer to function with custom guide RNA (gRNA) in human cells to establish an RNA-guided editing tool for facile, robust, and multiplexable human genome engineering.
- Type
- article
- Published
- 2013-01-03
- Cited by
- 9,073
- References
- 68
- Access
- Open access
- OpenAlex
- https://openalex.org/W2003171404
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:14055913
Keywords
CRISPR, Guide RNA, Biology, Genome editing, Cas9
References
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- The accessible chromatin landscape of the human genome
- Gene targeting of a disease-related gene in human induced pluripotent stem and embryonic stem cells
- Ieee Transactions On Cybernetics
- The CRISPR/Cas bacterial immune system cleaves bacteriophage and plasmid DNA
- Breaking the Code of DNA Binding Specificity of TAL-Type III Effectors
- Gene editing in human stem cells using zinc finger nucleases and integrase-defective lentiviral vector delivery
- Site-specific gene correction of a point mutation in human iPS cells derived from an adult patient with sickle cell disease.
- En route to ethical recommendations for gene transfer clinical trials.
- U6 promoter–driven siRNAs with four uridine 3′ overhangs efficiently suppress targeted gene expression in mammalian cells
- A Transcription Activator-Like Effector (TALE) Toolbox for Genome Engineering
- Highly efficient endogenous human gene correction using designed zinc-finger nucleases
- Expanded sequence dependence of thermodynamic parameters improves prediction of RNA secondary structure.
- Diverse CRISPRs Evolving in Human Microbiomes
- Cas9–crRNA ribonucleoprotein complex mediates specific DNA cleavage for adaptive immunity in bacteria
- Synthetic biology: applications come of age
- An epidemic of complex dysmorphologic syndromes in southeast Spain?
- Highly efficient gene targeting of expressed and silent genes in human ESCs and iPSCs using zinc finger nucleases
- A System for Stable Expression of Short Interfering RNAs in Mammalian Cells
- In vivo genome editing restores hemostasis in a mouse model of hemophilia
Cited by
- Programmable DNA cleavage in vitro by Cas9.
- DNA hydrolysis and genome editing applications of GIY-YIG family homing endonucleases
- Assaying break and nick-induced homologous recombination in mammalian cells using the DR-GFP reporter and Cas9 nucleases
- TAL effectors: highly adaptable phytobacterial virulence factors and readily engineered DNA targeting proteins
- Modeling human disease with pluripotent stem cells: from genome association to function.
- Evolutionary Dynamics of the Prokaryotic Adaptive Immunity System CRISPR-Cas in an Explicit Ecological Context
- Multiplex and homologous recombination-mediated plant genome editing via guide RNA/Cas9
- Engineering the Caenorhabditis elegans Genome Using Cas9-Triggered Homologous Recombination
- A variant CRISPR-Cas9 system adds versatility to genome engineering
- Genetic marking of sex using a W chromosome-linked transgene.
- Heritable gene-targeting with gRNA/Cas9 in rats
- The Presenilin-1 ΔE9 mutation results in reduced γ-secretase activity, but not total loss of PS1 function, in isogenic human stem cells
- Connecting signaling pathways underlying communication to ASD vulnerability.
- Genetic correction using engineered nucleases for gene therapy applications
- CRISPR/Cas9-mediated targeted mutagenesis in the liverwort Marchantia polymorpha L.
- DNA Replicons for Plant Genome Engineering[W][OPEN]
- Cas9 in close-up
- Genome-wide binding of the CRISPR endonuclease Cas9 in mammalian cells
- Heritable Genome Editing with CRISPR/Cas9 in the Silkworm, Bombyx mori
- Expansion of the CRISPR-Cas9 genome targeting space through the use of H1 promoter-expressed guide–RNAs
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