Comparison of solution hybridization efficiencies using alkaline phosphatase-labelled and 32P-labelled oligodeoxynucleotide probes.
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Summary
The hybridization efficiencies of oligonucleotide probes directly labelled with alkaline phosphatase and probes labelled with 32P were compared by quantitating the enzyme activity or radioactivity associated with hybridization targets over time by quantitative analysis of the time course and efficiency.
- Type
- article
- Published
- 1991-04-01
- Cited by
- 14
- References
- 13
- OpenAlex
- https://openalex.org/W1986385570
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:9533164
Keywords
Biology, Alkaline phosphatase, Biochemistry, Molecular biology, Enzyme
References
- A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
- Solution hybridization of crosslinkable DNA oligonucleotides to bacteriophage M13 DNA. Effect of secondary structure on hybridization kinetics and equilibria.
- Analysis of a major human chorionic somatomammotropin gene. Evidence for two functional promoter elements.
- A simple, rapid, and sensitive DNA assay procedure.
- Kinetics of renaturation of DNA.
- Preparation of oligodeoxynucleotide-alkaline phosphatase conjugates and their use as hybridization probes.
- Improved microfluorometric DNA determination in biological material using 33258 Hoechst.
- Rates of formation and thermal stabilities of RNA:DNA and DNA:DNA duplexes at high concentrations of formamide.
- Temperature‐jump kinetics of the dC‐G‐T‐G‐A‐A‐T‐T‐C‐G‐C‐G double helix containing a G·T base pair and the dC‐G‐C‐A‐G‐A‐A‐T‐T‐C‐G‐C‐G double helix containing an extra adenine
- A practical approach for quantitating specific mRNAs by solution hybridization.
- [105] Determination of DNA concentration with diphenylamine
Cited by
- Handbook of Analysis of Oligonucleotides and Related Products
- Simultaneous detection of two cystic fibrosis alleles using dual-label time-resolved fluorometry.
- The preparation of enzyme-labelled oligonucleotides by reductive amination
- Optimization of the rate of DNA hybridization and rapid detection of methicillin resistant Staphylococcus aureus DNA using fluorescence polarization.
- Rapid Detection of Complementary-and Mismatched DNA Sequences Using Fluorescence Polarization
- Quantitative rRNA-Targeted Solution-Based Hybridization Assay Using Peptide Nucleic Acid Molecular Beacons
- Cystic fibrosis: molecular approaches to diagnosis.
- Oligozymes. Oligonucleotide-enzyme conjugates.
- Oligonucleotide-enzyme conjugates.
- The SNAP System
- Enzyme-Labeled Oligonucleotides
- Detection of Cystic Fibrosis AF 508 Mutation by Anti-Double-Stranded DNA Antibody * !
- In Situ Hybridization for Peptide mRNA
- Purity Analysis and Molecular Weight Determination by Size Exclusion HPLC
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