The preparation of enzyme-labelled oligonucleotides by reductive amination

Keywords

Reductive amination, Chemistry, Conjugate, Oligonucleotide, Residue (chemistry)

References

• Non-isotopic in situ hybridization with digoxigenin and alkaline phosphatase labelled oligodeoxynucleotide probes. Applications in pituitary gland.A.M. McNicol, M. A. Farquharson, Edwin Walker — 1991
• Identification of enterotoxigenic Escherichia coli isolates with enzyme-labeled synthetic oligonucleotide probesPeter P. Medon, Janice A. Lanser, P R Monckton — 1988
• Chemiluminescent substrates for alkaline phosphatase: application to ultrasensitive enzyme-linked immunoassays and DNA probes.A. Paul Schaap, Homa Akhavan, L J Romano — 1989
• Fluorometric assay of serum acid or alkaline phosphatase, either in solution or on a semisolid surface.Bernd Rietz, George G. Guilbault — 1975
• Hybridisation detection of single nucleotide changes with enzyme labelled oligonucleotides.A M Alves, David A. Holland, Michael D. Edge — 1988
• Comparison of solution hybridization efficiencies using alkaline phosphatase-labelled and 32P-labelled oligodeoxynucleotide probes.Sheila Podell, William Maske, Elaine Ibañez — 1991
• Highly sensitive detection of DNA using enzyme-linked DNA-probe. 1. Colorimetric and fluorometric detection.Akira Murakami, Jun Tada, Koichi Yamagate — 1989
• Nonradioactive detection of vasopressin mRNA with in situ hybridization histochemistry.Frank Baldino, Jerry L. Ruth, Leonard G. Davis — 1989
• Distribution of Tyrosine Hydroxylase mRNA in the Rat Central Nervous System Visualized by Alkaline Phosphatase in situ Hybridization HistochemistryHiroshi Kiyama, P.C. Emson, Jerry L. Ruth — 1990
• Preparation of oligodeoxynucleotide-alkaline phosphatase conjugates and their use as hybridization probes.Edward Jablonski, Ellen W. Moomaw, Richard H. Tullis — 1986
• A comparison of non-radioisotopic hybridization assay methods using fluorescent, chemiluminescent and enzyme labeled synthetic oligodeoxyribonucleotide probes.Mickey S. Urdea, Brian D. Warner, J A Running — 1988
• The synthesis of polyamide-oligonucleotide conjugate molecules.Jim Haralambidis, Lucy Duncan, Karin Angus — 1990
• Sensitive non-radioisotopic in situ hybridization histochemistry: demonstration of tyrosine hydroxylase gene expression in rat brain and adrenal.Hiroshi Kiyama, P.C. Emson, Jerry L. Ruth — 1990
• Immobilization of DNA via oligonucleotides containing an aldehyde or carboxylic acid group at the 5' terminus.Jonathan N. Kremsky, Joseph L. Wooters, Joseph P. Dougherty — 1987
• Enzyme-linked synthetic oligonucleotide probes: non-radioactive detection of enterotoxigenic Escherichia coli in faecal specimensPeng Li, Peter P. Medon, Derek C. Skingle — 1987
• An in situ hybridization histochemistry method for the use of alkaline phosphatase-labeled oligonucleotide probes in small intestine.Hiroshi Kiyama, P.C. Emson — 1991
• Antibodies to carbohydrates: preparation of antigens by coupling carbohydrates to proteins by reductive amination with cyanoborohydride.Gary R. Gray — 1978
• An improved preparation and purification of oligonucleotide-alkaline phosphatase conjugates.Farmar Jg, María Teresita Castañeda — 1991

Cited by

• Attachment of Reporter and Conjugate Groups to the 3′ Termini of OligonucleotidesMarc M. Greenberg — 2000
• Use of carbonyl group addition--elimination reactions for synthesis of nucleic acid conjugates.Timofei S. Zatsepin, Dmitry A. Stetsenko, Michael J. Gait — 2005
• Synthesis of aldehyde-linked nucleotides and DNA and their bioconjugations with lysine and peptides through reductive amination.Veronika Raindlová, Radek Pohl, Michal Hocek — 2012

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