A simple, rapid, and sensitive DNA assay procedure.
Explore this paper's citation graph
Summary
The dissociation of chromatin is critical to accurate determinations of DNA in biological materials using this method, which can detect as little as 10 ng of DNA with rather unsophisticated instrumentation.
- Type
- article
- Published
- 1980-03-01
- Cited by
- 4,884
- References
- 23
- OpenAlex
- https://openalex.org/W1977779461
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:38386440
Keywords
Simple (philosophy), DNA, Chemistry, Chromatography, Computational biology
References
- Drugs and nucleic acids
- Intercalative binding of ellipticine to DNA.
- Variation of the supercoils in closed circular DNA by binding of antibiotics and drugs: evidence for molecular models involving intercalation.
- Chromosomal protein interactions in chromatin and with DNA.
- Stopped-flow kinetic studies of actinomycin binding to DNAs.
- Physicochemical properties of complexes between deoxyribonucleic acid and antibiotics which affect ribonucleic acid synthesis (actinomycin, daunomycin, cinerubin, nogalamycin, chormomycin, mithramycin, and olivomycin).
- Antibiotics and nucleic acids.
- Assay for nanogram quantities of DNA in cellular homogenates.
- Mobility and the Restriction of Mobility of Plasma Membrane Lectin-Binding Components
- Simple and rapid fluorimetric method for DNA microassay.
- A rapid fluorometric method for the determination of DNA in human semen.
- Staining of constitutive heterochromatin in mammalian chromosomes with a new fluorochrome.
- Improvements in the ethidium bromide method for direct fluorometric estimation of DNA and RNA in cell and tissue homogenates.
- Modifications of the diphenylamine reaction giving increased sensitivity and simplicity in the estimation of DNA.
- Base Specificity in the Interaction of Polynucleotides with Antibiotic Drugs
- The requirement of intact double helical structure for primary binding in quinacrine-nucleic acid complexes.
- Cell-Cycle Analysis in 20 Minutes
- Evidence for heterogeneity in heterochromatin of Drosophila melanogaster
- The interaction of deoxyribonucleic acid and acridine orange.
- Estimation of accessibility of DNA in chromatin from fluorescence measurements of electronic excitation energy transfer
Cited by
- Fetal topography of bovine rhodopsin mRNA suggests retinotopographically determined gene expression.
- Growth on type I collagen promotes expression of the osteoblastic phenotype in human osteosarcoma MG‐63 cells
- Effect of hypophysectomy on rat preadipocyte replication and differentiation.
- Fluorimetric DNA assay for cell growth estimation.
- Determination of DNA via Resonance Light Scattering technique using new probes derived from ruthenium ligands complexes and investigation of their binding mode
- Adsorption of plasmid DNA to mineral surfaces and protection against DNase I
- Cellular alterations of parotid gland of rats with acute pancreatitis induced by cerulein
- Rapid resolution of duck hepatitis B virus infections occurs after massive hepatocellular involvement
- Identification of components in Fusobacterium nucleatum chemostat-culture supernatants that are potent inhibitors of human gingival fibroblast proliferation.
- Follicle-stimulating hormone increases concentrations of messenger ribonucleic acid encoding cytochrome P450 cholesterol side-chain cleavage enzyme in primary cultures of porcine granulosa cells.
- Time-Related Distribution Profiles of Sulfated Glycosaminoglycans in Cells, Cell Surfaces, and Media of Cultured Rat Liver Fat-Storing Cells
- Differential regulation of gonadotropin subunit gene expression by gonadotropin-releasing hormone pulse amplitude in female rats.
- The Zellweger syndrome: deficient conversion of docosahexaenoic acid (22:6(n-3)) to eicosapentaenoic acid (20:5(n-3)) and normal delta 4-desaturase activity in cultured skin fibroblasts.
- The bacteriophage Mu gem gene: a positive regulator of the C operon required for normal levels of late transcription.
- Differential poly(ADP-ribose) metabolism in repair-proficient and repair-deficient murine lymphoma cells.
- Lead inhibits the basal and stimulated responses of a rat osteoblast-like cell line ROS 17/2.8 to 1 alpha,25-dihydroxyvitamin D3 and IGF-I.
- Ethanol alters astrocyte development: A study of critical periods using primary cultures
- The interaction of human papillary and reticular fibroblasts and human keratinocytes in the contraction of three-dimensional floating collagen lattices.
- Oxytocin and progesterone release from bovine corpus luteal cells in culture: effects of insulin-like growth factor I, insulin, and prostaglandins.
- Measurement of nuclear oestrogen receptors in human breast tumours.
Related papers
- The [0-Simple] Simple Subsemigroups of Nonnegative Matrices
- Fundamental relations in simple and 0-simple semihypergroups of small size
- Book Review: IV. Pastorial — Practical Studies: Simple Sermons for Times like These, Simple Sermons on Evangelistic Themes, Simple Talks for Christian Workers, Simple Sermons on Prophetic Themes, Simple Sermons about Jesus Christ, Simple Sermons on Heaven, Hell, and Judgment, Simple Sermons on the Ten Commandments, Simple Sermons for a Sinful Age, Simple Sermons on the Seven Churches of Revelation
- Simple Containers for Simple Hypergraphs
- Simple endotrivial modules for quasi-simple groups
- Simple Concepts and Simple Expressions