Use of T7 RNA polymerase to direct expression of cloned genes.
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- Type
- article
- Published
- 1990-01-01
- Cited by
- 6,253
- References
- 21
- Access
- Open access
- OpenAlex
- https://openalex.org/W1873199501
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:5177351
Keywords
T7 RNA polymerase, Polymerase, Molecular biology, Gene, Biology
References
- Utilization of bacteriophage T7 late promoters in recombinant plasmids during infection.
- Characterization of T7-specific ribonucleic acid polymerase. 1. General properties of the enzymatic reaction and the template specificity of the enzyme.
- Bacteriophage T7 lysozyme is an N-acetylmuramyl-L-alanine amidase.
- The purification of eukaryotic polypeptides synthesized in Escherichia coli.
- DNA sequence for a low-level promoter of the lac repressor gene and an ‘up’ promoter mutation
- New RNA Polymerase from Escherichia coli infected with Bacteriophage T7
- Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes.
- Genetic mapping of a mutation that causes ribonucleases III deficiency in Escherichia coli
- Complete nucleotide sequence of bacteriophage T7 DNA and the locations of T7 genetic elements.
- Nucleotide sequence of the kanamycin resistance transposon Tn903.
- Vectors for selective expression of cloned DNAs by T7 RNA polymerase.
- Targeting bacteriophage T7 RNA polymerase to the mammalian cell nucleus.
- Sequence of the lacI gene
- Cleavage within an RNase III site can control mRNA stability and protein synthesis in vivo.
- Revised sequence of the tetracycline-resistance gene of pBR322.
- The role of antisense RNA in gene regulation.
- ompT encodes the Escherichia coli outer membrane protease that cleaves T7 RNA polymerase during purification
- T7 lysozyme inhibits transcription by T7 RNA polymerase.
- Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid
- Distinguishing between mechanisms of eukaryotic transcriptional activation with bacteriophage T7 RNA polymerase.
Cited by
- High-throughput cloning for proteomics research.
- Alternative promoter usage of the Fos‐responsive gene Fit‐1 generates mRNA isoforms coding for either secreted or membrane‐bound proteins related to the IL‐1 receptor.
- Purification, cloning, and RXR identity of the HeLa cell factor with which RAR or TR heterodimerizes to bind target sequences efficiently.
- Glycogen synthase kinase‐3 and the Alzheimer‐like state of microtubule‐associated protein tau
- Localization of the virus neutralizing and hemagglutinin epitopes of E1 glycoprotein of rubella virus
- Mutagenesis of the L protein encoded by Bunyamwera virus and production of monospecific antibodies.
- Polyhydroxyalkanoate production in recombinant Escherichia coli.
- Kinesin-related cut 7 protein associates with mitotic and meiotic spindles in fission yeast
- Replication origin mutations affecting binding of pSC101 plasmid-encoded Rep initiator protein
- HIV protease mutations leading to reduced inhibitor susceptibility.
- Gene expression from multicopy T7 promoter vectors proceeds at single copy rates in the absence of T7 RNA polymerase.
- Definition of the inhibitory domain of smooth muscle myosin light chain kinase by site-directed mutagenesis.
- Reconstitution of electron transport in photosystem I with PsaC and PsaD proteins expressed in Escherichia coli
- Biosynthesis of chlorosome proteins is not inhibited in acetylene-treated cultures of Chlorobium vibrioforme
- Screening and optimizing protein production in E. coli.
- Cellular factors required for papillomavirus DNA replication
- Assembly of tobacco mosaic virus and TMV-like pseudovirus particles in Escherichia coli.
- An extended -10 promoter alone directs transcription of the DpnII operon of Streptococcus pneumoniae.
- Examination of recombinant truncated mature human fibroblast collagenase by mass spectrometry: identification of differences with the published sequence and determination of stable isotope incorporation.
- N-terminal variants of fatty acid-binding protein from bovine heart overexpressed in Escherichia coli.
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- A mutant T7 phage promoter is specifically transcribed by T7-RNA polymerase in mammalian cells.
- Four T7 RNA polymerase promoters contain an identical 23 bp sequence.
- Comparison of Polymerase Subunits from Double-Stranded RNA Bacteriophages
- Structure of T7 RNA polymerase complexed to the transcriptional inhibitor T7 lysozyme