A mutant T7 phage promoter is specifically transcribed by T7-RNA polymerase in mammalian cells.
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Summary
It is demonstrated that nuclear-factor-including components of the TFIID fraction, bind to the T7 promoter and inhibit transcription by T7-RNA polymerase.
- Type
- article
- Published
- 1993-10-01
- Cited by
- 27
- References
- 22
- Access
- Open access
- OpenAlex
- https://openalex.org/W1989631604
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:25919667
Keywords
T7 RNA polymerase, Molecular biology, Transcription factor II D, RNA polymerase I, RNA-dependent RNA polymerase
References
- Watch without Mother
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- A new technique for the assay of infectivity of human adenovirus 5 DNA.
- Discrimination between bacteriophage T3 and T7 promoters by the T3 and T7 RNA polymerases depends primarily upon a three base-pair region located 10 to 12 base-pairs upstream from the start site.
- YY1 is an initiator sequence-binding protein that directs and activates transcription in vitro
- A downstream initiation element required for efficient TATA box binding and in vitro function of TFIID
- Transcriptional activation by Sp1 as directed through TATA or initiator: specific requirement for mammalian transcription factor IID.
- Complete nucleotide sequence of bacteriophage T7 DNA and the locations of T7 genetic elements.
- Eukaryotic transient-expression system based on recombinant vaccinia virus that synthesizes bacteriophage T7 RNA polymerase.
- High level gene expression in mammalian cells by a nuclear T7-phase RNA polymerase.
- A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.
- New mammalian expression vectors
- Functional significance of sequences following the TATA box of an immunoglobulin promoter studied by random mutagenesis
- Nucleotide sequence of the gene for bacteriophage T7 RNA polymerase.
- Metallothionein-human GH fusion genes stimulate growth of mice.
- Targeting bacteriophage T7 RNA polymerase to the mammalian cell nucleus.
- Regulated expression of foreign genes in mammalian cells under the control of coliphage T3 RNA polymerase and lac repressor.
- Cooperative interaction of an initiator-binding transcription initiation factor and the helix–loop–helix activator USF
- Firefly luciferase gene: structure and expression in mammalian cells
Cited by
- Retrohoming of a Mobile Group II Intron in Human Cells Suggests How Eukaryotes Limit Group II Intron Proliferation
- Elimination of hepatitis C virus RNA in infected human hepatocytes by adenovirus-mediated expression of ribozymes
- Selection of efficient cleavage sites in target RNAs by using a ribozyme expression library
- Mechanisms of replication-deficient vaccinia virus/T7 RNA polymerase hybrid expression: effect of T7 RNA polymerase levels and alpha-amanitin.
- Knock down of gfp and no tail expression in zebrafish embryo by in vivo-transcribed short hairpin RNA with T7 plasmid system.
- T7 RNA polymerase drives transcription of a reporter gene from T7 promoter, but engenders post-transcriptional silencing of expression
- Adenoviral gene transfer of the human V2 vasopressin receptor improves contractile force of rat cardiomyocytes.
- Enhancement of phospholipase D activity following baculovirus and adenovirus infection in Sf9 and COS-7 cells.
- Reconstitution of mutant V2 vasopressin receptors by adenovirus-mediated gene transfer. Molecular basis and clinical implication.
- Evidence that the 5′-End Cap Structure Is Essential for Encapsidation of Hepatitis B Virus Pregenomic RNA
- Na+/Ca2+ exchanger overexpression impairs frequency- and ouabain-dependent cell shortening in adult rat cardiomyocytes.
- Reactivation of the Previously Silenced Cytomegalovirus Major Immediate-Early Promoter in the Mouse Liver: Involvement of NFκB
- Evaluation of HBV promoters for use in hepatic gene therapy.
- A Processive Protein Chimera Introduces Mutations Across Defined DNA Regions In Vivo
- Snake Deltavirus Utilizes Envelope Proteins of Different Viruses To Generate Infectious Particles
- In vivo diversification of target genomic sites using processive T7 RNA polymerase-base deaminase fusions blocked by RNA-guided dCas9
- In vivo diversification of target genomic sites using processive base deaminase fusions blocked by dCas9
- Short ‘1.2× genome’ infectious clone initiates deltavirus replication in Boa constrictor cells
- Expression systems
- Short ‘1.2× Genome’ Infectious Clone Initiates Kolmiovirid Replication in Boa constrictor Cells
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