High-throughput cloning for proteomics research.
Explore this paper's citation graph
Summary
This chapter describes the creation of an LIC-compatible vector, with tips on how to make any vector LIC-enabled, and includes a protocol for generating high-quality linearized vector template for the LIC reaction.
- Type
- article
- Published
- 2005-01-01
- Cited by
- 33
- References
- 7
- Access
- Open access
- OpenAlex
- https://openalex.org/W418398
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:21247887
Keywords
Cloning (programming), Vector (molecular biology), Computer science, Insert (composites), Throughput
References
- Rapid, reliable ligation-independent cloning of PCR products using modified plasmid vectors.
- Use of T7 RNA polymerase to direct expression of cloned genes.
- Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes.
- Vectors for selective expression of cloned DNAs by T7 RNA polymerase.
- Ligation-independent cloning of PCR products (LIC-PCR).
- Minimal length requirement of the single-stranded tails for ligation-independent cloning (LIC) of PCR products.
- A new vector for high-throughput, ligation-independent cloning encoding a tobacco etch virus protease cleavage site.
Cited by
- Flexi Vector Cloning
- Cloning, expression, purification and crystallographic studies of galectin-11 from domestic sheep (Ovis aries).
- Control of carotenoid biosynthesis through a heme-based cis-trans isomerase
- Biochemical and EPR-Spectroscopic Investigation into Heterologously Expressed Vinyl Chloride Reductive Dehalogenase (VcrA) from Dehalococcoides mccartyi Strain VS
- Two Neonatal Diabetes Mutations on Transmembrane Helix 15 of SUR1 Increase Affinity for ATP and ADP at Nucleotide Binding Domain 2*
- Analysis of DNA relaxation and cleavage activities of recombinant Mycobacterium tuberculosis DNA topoisomerase I from a new expression and purification protocol
- Enzyme Free Cloning for high throughput gene cloning and expression
- Expression and characterization of the Neurospora crassa endoglucanase GH5-1.
- Crystal structures of Phanerochaete chrysosporium pyranose 2-oxidase suggest that the N-terminus acts as a propeptide that assists in homotetramer assembly
- A monoclinic crystal form of casein kinase 1 δ.
- A kinetically trapped intermediate of FK506 binding protein forms in vitro: chaperone machinery dominates protein folding in vivo.
- Residues of E. coli topoisomerase I conserved for interaction with a specific cytosine base to facilitate DNA cleavage
- High-resolution crystal structure of a polyextreme GH43 glycosidase from Halothermothrix orenii with α-l-arabinofuranosidase activity
- The Strictly Conserved Arg-321 Residue in the Active Site of Escherichia coli Topoisomerase I Plays a Critical Role in DNA Rejoining*
- Inhibition of Mg2+ binding and DNA religation by bacterial topoisomerase I via introduction of an additional positive charge into the active site region
- Asp to Asn substitution at the first position of the DxD TOPRIM motif of recombinant bacterial topoisomerase I is extremely lethal to E. coli
- A Versatile Set of Ligation-Independent Cloning Vectors for Functional Studies in Plants1[C][W][OA]
- Structural basis for the MukB‐topoisomerase IV interaction and its functional implications in vivo
- Rapid Cloning For Protein Crystallography Using Type IIS Restriction Enzymes
- Estudos funcionais e estruturais de enzimas frutosiltransferases das famílias 32 e 68 de hidrolases de glicosídeos
Related papers
- Engineering a Minimal 1185 Bp Cloning Vector from a Puc18 Plasmid Backbone with an Extended Multiple Cloning Site
- Engineering a minimal cloning vector from a pUC18 plasmid backbone with an extended multiple cloning site.
- Engineering a Minimal Cloning Vector from a pUC18 Plasmid Backbone with an Extended Multiple Cloning Site
- Construction of a High Efficiency PCR Products Cloning T Vector Using pGEM-5zf (+)
- Construction of T-vector for PCR cloning
- Cloning of transgenes CRY 1A(c) and Cry 2Ab in BT- Cotton (BG-II) by TOPOTA cloning kit method
- Construction of a T vector and cloning of a differentially displayed fragment of wheat
- A selective lambda phage cloning vector with automatic excision of the insert in a plasmid.