Characterization of T7-specific ribonucleic acid polymerase. 1. General properties of the enzymatic reaction and the template specificity of the enzyme.
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Summary
It is suggested that T7 RNA polymerase requires a specific promoter site on DNA for effective transcription; this site is different from that used by bacterialRNA polymerase and may be rich in cytosine and thymidine residues.
- Type
- article
- Published
- 1973-03-25
- Cited by
- 152
- References
- 0
- Access
- Open access
- OpenAlex
- https://openalex.org/W1487026581
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:29805160
Keywords
Polymerase, Chemistry, Biochemistry, DNA
References
No references recorded for this paper.
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- A chromosomally encoded T7 RNA polymerase-dependent gene expression system for Corynebacterium glutamicum: construction and comparative evaluation at the single-cell level
- Optimisation of a transcription-translation coupled in vitro system
- Mapping of Class II Promoter Sites Utilized In Vitro by T7-Specific RNA Polymerase on Bacteriophage T7 DNA
- Use of T7 RNA polymerase in an optimized Escherichia coli coupled in vitro transcription-translation system. Application in regulatory studies and expression of long transcription units.
- Probing the mechanics of the complete DNA transcription cycle in real-time using optical tweezers.
- Regulation of gene expression in prokaryotic organisms.
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- Iron acquisition by Shigella dysenteriae and Shigella flexneri
- Abortive products as initiating nucleotides during transcription by T7 RNA polymerase.
- Coupled-enzymatic assays for the rate and mechanism of DNA site exposure in a nucleosome.
- The RNA Polymerase of Marine Cyanophage Syn5*
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