Effects of mutations in poliovirus 3Dpol on RNA polymerase activity and on polyprotein cleavage
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Summary
A series of short insertion mutations was introduced into the poliovirus gene for 3Dpol at a number of different locations, and when substituted for wild-type sequences in a full-length, infectious cDNA and tested for infectivity, all 3D mutants were nonviable.
- Type
- article
- Published
- 1989-11-01
- Cited by
- 55
- References
- 52
- Access
- Open access
- OpenAlex
- https://openalex.org/W1793294936
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:30408270
Keywords
Biology, RNA polymerase, Mutant, Polymerase, RNA-dependent RNA polymerase
References
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- Expression of enzymatically active poliovirus RNA-dependent RNA polymerase in Escherichia coli.
- A simple method for displaying the hydropathic character of a protein.
- In vitro molecular genetics as a tool for determining the differential cleavage specificities of the poliovirus 3C proteinase.
- A rapid, sensitive method for detection of alkaline phosphatase-conjugated anti-antibody on Western blots.
- Poliovirus polypeptide precursors: expression in vitro and processing by exogenous 3C and 2A proteinases.
- A point mutation in the poliovirus polymerase gene determines a complementable temperature-sensitive defect of RNA replication.
- Rapid and sensitive colorimetric method for visualizing biotin-labeled DNA probes hybridized to DNA or RNA immobilized on nitrocellulose: Bio-blots.
Cited by
- Biochemical characterization of rhinovirus RNA-dependent RNA polymerase.
- Picornavirus protein processing--enzymes, substrates, and genetic regulation.
- Pathogenesis of Theiler's Murine Encephalomyelitis Virus
- Replication of poliovirus in Xenopus oocytes requires two human factors.
- Poliovirus RNA replication.
- Molecular characterization of the saguaro cactus virus RNA-dependent RNA polymerase and capsid protein
- Purification and characterization of poliovirus polypeptide 3CD, a proteinase and a precursor for RNA polymerase
- A mutation in the RNA polymerase of poliovirus type 1 contributes to attenuation in mice
- Association of heat shock protein 70 with enterovirus capsid precursor P1 in infected human cells
- Regulation of Gene Expression in Animal Viruses
- Reverse genetics of picornaviruses.
- Identification of terminal adenylyl transferase activity of the poliovirus polymerase 3Dpol
- Clustered charged-to-alanine mutagenesis of poliovirus RNA-dependent RNA polymerase yields multiple temperature-sensitive mutants defective in RNA synthesis
- Enzymatic activity of poliovirus RNA polymerase mutants with single amino acid changes in the conserved YGDD amino acid motif
- trans rescue of a mutant poliovirus RNA polymerase function
- Temperature-sensitive polioviruses containing mutations in RNA polymerase.
- Recent advances in research on human enterovirus 71
- Distinct Conformations of a Putative Translocation Element in Poliovirus Polymerase
- Modulation of the RNA Binding and Protein Processing Activities of Poliovirus Polypeptide 3CD by the Viral RNA Polymerase Domain*
- Trans-dominant inhibition of RNA viral replication can slow growth of drug-resistant viruses
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