Expression of enzymatically active poliovirus RNA-dependent RNA polymerase in Escherichia coli.
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Summary
The availability of a recombinant, enzymatically active poliovirus RNA polymerase provides a system in which the authors can precisely delineate the role this enzyme plays in the regulation of poliov virus replication.
- Type
- article
- Published
- 1987-09-01
- Cited by
- 46
- References
- 1
- Access
- Open access
- OpenAlex
- https://openalex.org/W1974233081
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:13436964
Keywords
RNA-dependent RNA polymerase, RNA polymerase, Polymerase, RNA polymerase I, Biology
References
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Cited by
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- Biochemical characterization of rhinovirus RNA-dependent RNA polymerase.
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- Characterization of the viral proteins involved in the RNA replication of cowpea mosaic virus
- The roles of turnip yellow mosaic virus genes in virus replication
- RNA duplex unwinding activity of poliovirus RNA-dependent RNA polymerase 3Dpol
- Coupled translation and replication of poliovirus RNA in vitro: synthesis of functional 3D polymerase and infectious virus
- Coinfection with recombinant vaccinia viruses expressing poliovirus P1 and P3 proteins results in polyprotein processing and formation of empty capsid structures
- Expression, purification, and properties of recombinant encephalomyocarditis virus RNA-dependent RNA polymerase
- Expression and subcellular localization of poliovirus VPg-precursor protein 3AB in eukaryotic cells: evidence for glycosylation in vitro
- Mutation of lysine residues in the nucleotide binding segments of the poliovirus RNA-dependent RNA polymerase
- Clustered charged-to-alanine mutagenesis of poliovirus RNA-dependent RNA polymerase yields multiple temperature-sensitive mutants defective in RNA synthesis
- Yeast cells are incapable of translating RNAs containing the poliovirus 5' untranslated region: evidence for a translational inhibitor
- Enzymatic activity of poliovirus RNA polymerase mutants with single amino acid changes in the conserved YGDD amino acid motif
- Effects of mutations in poliovirus 3Dpol on RNA polymerase activity and on polyprotein cleavage
- Purification and properties of poliovirus RNA polymerase expressed in Escherichia coli
- Cleavage site mutations in the encephalomyocarditis virus P3 region lethally abrogate the normal processing cascade
- RNA-dependent RNA Polymerase Activity of the Soluble Recombinant Hepatitis C Virus NS5B Protein Truncated at the C-terminal Region*
- Temperature-sensitive polioviruses containing mutations in RNA polymerase.
- What Is the Role of Motif D in the Nucleotide Incorporation Catalyzed by the RNA-dependent RNA Polymerase from Poliovirus?
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