A rapid, sensitive method for detection of alkaline phosphatase-conjugated anti-antibody on Western blots.
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Summary
A rapid, sensitive method has been developed to detect antibody-antigen complexes on "Western blots" by using Tween 20 to separate and blot the antigens onto nitrocellulose.
- Type
- article
- Published
- 1984-01-01
- Cited by
- 2,056
- References
- 13
- OpenAlex
- https://openalex.org/W1984367265
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:39024481
Keywords
Nitrocellulose, Alkaline phosphatase, Chemistry, Western blot, Molecular biology
References
- Studies on gonococcus infection. XVIII. 125I-labeled peptide mapping of the major protein of the gonococcal cell wall outer membrane
- Enzyme-linked immunosorbent assay, Elisa. 3. Quantitation of specific antibodies by enzyme-labeled anti-immunoglobulin in antigen-coated tubes.
- Application of the nitrocellulose transfer technique and alkaline phosphatase conjugated anti-immunoglobulin for determination of the specificity of monoclonal antibodies to protein mixtures.
- The use of Tween 20 as a blocking agent in the immunological detection of proteins transferred to nitrocellulose membranes.
- Detection of antigens and antibodies by an immuno-peroxidase method applied on thin longitudinal sections of SDS-polyacrylamide gels.
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
- A Simple and Reproducible Method for Crossed Immunoelectrofocusing
- "Western blotting": electrophoretic transfer of proteins from sodium dodecyl sulfate--polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A.
- Coupling of Enzymes to Antibodies and Antigens
- Effects of proteolytic enzymes on the outer membrane proteins of Neisseria gonorrhoeae
- Purification and partial characterization of the major outer membrane protein of Neisseria gonorrhoeae
- Filter affinity transfer. A new technique for the in situ identification of proteins in gels.
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- Disulphide bridge formation in the periplasm of Escherichia coli: β‐lactamase::human lgG3 hinge fusions as a model system
- Phosphorylation of chicken oviduct progesterone receptor by cAMP-dependent protein kinase.
- A comparison of the intracellular distribution of mu-calpain, m-calpain, and calpastatin in proliferating human A431 cells.
- Restricted tissue distribution of a 37-kD possible adherens junction protein
- Copper-induced expression, cloning, and regulatory studies of the plastocyanin gene from the cyanobacterium Synechocystis sp. PCC 6803
- Secretion and membrane integration of a filamentous phage-encoded morphogenetic protein.
- Astroglia‐released neurite growth‐inducing activity for embryonic hippocampal neurons is associated with laminin bound in a sulfated complex and free fibronectin
- Translation of merD in Tn21
- Comprehensive polypeptide analysis of microdissected rat brain areas: combining 2-dimensional gel electrophoresis with 2-dimensional HPLC and immunoanalysis and sequencing procedures.
- Regulation of rat olfactory glutathione S-transferase expression. Investigation of sex differences, induction, and ontogenesis.
- Secretion of Escherichia coli chloramphenicol acetyltransferase by mammalian cells.
- Immunolocalization of laminin during estrogen‐induced differentiation of quail oviduct epithelial cells
- Alteration of the Fe protein of nitrogenase by oxygen in the cyanobacterium Anabaena sp. strain CA
- Characterization and specificity of antibodies to protein I of Neisseria gonorrhoeae produced by injection with various protein I- adjuvant preparations
- Expression of staphylococcal enterotoxin C1 in Escherichia coli
- Isolated bovine myocardial sarcolemma and sarcoplasmic reticulum vesicles contain tightly bound calcium-dependent protease inhibitor.
- Fluorescent monitoring of proteins during sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western blotting.
- Bacteriocuprein superoxide dismutases in pseudomonads
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