Metabolic labeling and click chemistry detection of glycoprotein markers of mesenchymal stem cell differentiation.
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Summary
The results demonstrate highly sensitive labeling of O-linked, sialic acid-containing, and O-GlcNAc modified proteins in all cell types without affecting cell growth or morphology.
- Type
- article
- Published
- 2011-01-01
- Cited by
- 32
- References
- 14
- OpenAlex
- https://openalex.org/W24560154
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:38339050
Keywords
Glycoprotein, Chemistry, Sialic acid, Biochemistry, Click chemistry
References
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- A stepwise huisgen cycloaddition process: copper(I)-catalyzed regioselective "ligation" of azides and terminal alkynes.
- Osteo-transcriptomics of human mesenchymal stem cells: accelerated gene expression and osteoblast differentiation induced by vitamin D reveals c-MYC as an enhancer of BMP2-induced osteogenesis.
- Multilineage potential of adult human mesenchymal stem cells.
- Fibroblast precursors in normal and irradiated mouse hematopoietic organs.
- Click Chemistry: Diverse Chemical Function from a Few Good Reactions
- PDGF, TGF- (cid:1) , and FGF signaling is important for differentiation and growth of mesenchymal stem cells (MSCs): transcriptional profiling can identify markers and signaling pathways important in differentiation of MSCs into adipogenic, chondrogenic, and osteogenic lineages
Cited by
- Undetectable histone O-GlcNAcylation in mammalian cells
- The Utilities of Chemical Reactions and Molecular Tools for O‐GlcNAc Proteomic Studies
- Novel Positively Charged Nanoparticle Labeling for In Vivo Imaging of Adipose Tissue-Derived Stem Cells
- Myosin IIa activation is crucial in breast cancer derived galectin-1 mediated tolerogenic dendritic cell differentiation.
- Structural analysis of glycoprotein sialylation – Part I: pre-LC-MS analytical strategies
- Glycoproteomic strategies: From discovery to clinical application of cancer carbohydrate biomarkers
- Proteome wide purification and identification of O-GlcNAc modified proteins using Click chemistry and mass spectrometry
- Engulfment and clearance of apoptotic cells based on a GlcNAc-binding lectin-like property of surface vimentin.
- Cell Labeling and Tracking Method without Distorted Signals by Phagocytosis of Macrophages
- Glycoengineered mesenchymal stem cells as an enabling platform for two-step targeting of solid tumors.
- Bioorthogonal Copper Free Click Chemistry for Labeling and Tracking of Chondrocytes In Vivo.
- Metabolisches Glykoengineering mit N‐Acyl‐Seiten‐ ketten‐modifizierten Mannosaminen
- Metabolic Glycoengineering with N-Acyl Side Chain Modified Mannosamines.
- Combined Antibody/Lectin-Enrichment Identifies Extensive Changes in the O-GlcNAc Sub-proteome Upon Oxidative Stress
- HIV-1 Proteomics
- Seasonal and flight-related variation of galectin expression in heart, liver and flight muscles of yellow-rumped warblers (Setophaga coronata)
- Studies towards the proteome-wide detection, identification and quantification of protein glycosylation
- Practical Considerations, Challenges, and Limitations of Bioconjugation via Azide-Alkyne Cycloaddition
- Metabolic labeling of HIV-1 envelope glycoprotein gp120 to elucidate the effect of gp120 glycosylation on antigen uptake
- Safety and Optimization of Metabolic Labeling of Endothelial Progenitor Cells for Tracking
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