Determination of transcription factor isoelectric point by two-dimensional native isoelectric focusing and electrophoretic mobility shift analysis.

Carlos Climent Durán · Analytical Biochemistry · 1992

Summary

A rapid, sensitive, and inexpensive assay is described for the determination of isoelectric points of native transcription factors derived from cell nuclei, applicable to any transcription factor which binds DNA specifically and may be used to identify changes in surface charge characteristics which occur as a result of alternative splicing events and/or following transcription factor post-translational modification(s).

Type: article
Published: 1992-10-01
Cited by: 3
References: 18
DOI: https://doi.org/10.1016/s0003-2697(05)80032-6
PubMed: https://pubmed.ncbi.nlm.nih.gov/1456433/
OpenAlex: https://openalex.org/W1456433
Semantic Scholar: https://api.semanticscholar.org/CorpusID:36966392

Keywords

Psychology

References

A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.Richard W. McEnally, Rebecca B. Todd — 1976
High resolution two-dimensional electrophoresis of proteins.Patrick H. O’Farrell — 1975
Identification of an octamer-binding site in the mouse kappa light-chain immunoglobulin enhancerA Currie, Robert G. Roeder — 1989
Differential transcriptional activation by Oct-1 and Oct-2: interdependent activation domains induce Oct-2 phosphorylation.Masafumi Tanaka, Winship Herr — 1990
Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei.John David Dignam, Russell M. Lebovitz, R G Roeder — 1983
A lymphoid-specific protein binding to the octamer motif of immunoglobulin genesLouis M. Staudt, Harinder Singh, Ranjan Sen — 1986
Promoter-selective activation domains in Oct-1 and Oct-2 direct differential activation of an snRNA and mRNA promoter.Masafumi Tanaka, Jiann-Shiun Lai, Winship Herr — 1992
Equilibria and kinetics of lac repressor-operator interactions by polyacrylamide gel electrophoresis.Michael G. Fried, Donald M. Crothers — 1981
Mitotic phosphorylation of the Oct-1 homeodomain and regulation of Oct-1 DNA binding activity.Neil Segil, Susan B. Roberts, N Heintz — 1991
Differential phosphorylation of the transcription factor Oct1 during the cell cycleSusan B. Roberts, Neil Segil, Nathaniel Heintz — 1991
Properties of a CCAAT box-binding protein.Rob Hooft van Huijsduijnen, Jacques Bollekens, Alper Döm — 1987
Transcriptional regulation in mammalian cells by sequence-specific DNA binding proteins.Pamela J. Mitchell, Robert Tjian — 1989
Purification and characterization of OTF-1, a transcription factor regulating cell cycle expression of a human histone H2b gene.Colin Fletcher, Nathaniel Heintz, Robert G. Roeder — 1987
Multiple Oct2 isoforms are generated by alternative splicingThomas Wirth, Annette Priess, Amd Annweiler — 1991
Eukaryotic transcriptional regulatory proteins.Peter F. Johnson, Steven L. McKnight — 1989
The ubiquitous octamer-binding protein Oct-1 contains a POU domain with a homeo box subdomain.Richard A. Sturm, Gautam Das, Winship Herr — 1988
Co‐evolution from yeast to mouse: cDNA cloning of the two NF‐Y (CP‐1/CBF) subunits.Rob Hooft van Huijsduijnen, X. Y. Li, Diane Black — 1990

Cited by

Contributions of mass spectrometry in the study of nucleic acid-binding proteins and of nucleic acid-protein interactions.Filippo Rusconi, François Guillonneau, Danièle Praseuth — 2002
Other than IPG‐DALT: 2‐DE variantsIngrid Miller, Ivano Eberini, Elisabetta Gianazza — 2010
A fiber-optic refractive index sensor detects the isoelectric point of gelatinLin Na Zhang, Bao Kai Zhang, Hong Yan Lei — 2018

Related papers

No related papers recorded.