Equilibria and kinetics of lac repressor-operator interactions by polyacrylamide gel electrophoresis.
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Summary
Gel electrophoresis in studies of equilibrium binding, site distribution, and kinetics of protein-DNA interactions found that binding to the so-called third operator site (03) is 15-18 fold weaker than operator binding, and that the binding reactions with the first and third operators are uncoupled, implying that there is no communication between the sites.
- Type
- article
- Published
- 1981-12-11
- Cited by
- 2,121
- References
- 0
- Access
- Open access
- OpenAlex
- https://openalex.org/W2004563262
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:10579279
Keywords
Repressor, Lac repressor, Operator (biology), Biology, Kinetics
References
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