Leginon: an automated system for acquisition of images from vitreous ice specimens.
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Summary
A system to automatically acquire cryo-electron micrographs is developed designed to emulate all of the decisions and actions of a highly trained microscopist in collecting data from a vitreous ice specimen.
- Type
- article
- Published
- 2000-10-01
- Cited by
- 329
- References
- 28
- OpenAlex
- https://openalex.org/W2095057795
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:2503225
Keywords
Magnification, Autofocus, Focus (optics), Computer science, Computer graphics (images)
References
- Introductory techniques for 3-D computer vision
- Practical autoalignment of transmission electron microscopes
- Automated microscopy.
- Automation of specimen selection and data acquisition for protein electron crystallography
- Electron scattering in ice and organic materials
- Towards automatic electron tomography. II. Implementation of autofocus and low-dose procedures
- Digitally collected cryo‐electron micrographs for single particle reconstruction
- Improving the positional accuracy of the goniometer on the Philips CM series TEM.
- A model for the microtubule-Ncd motor protein complex obtained by cryo-electron microscopy and image analysis.
- Electron microscopy of frozen biological suspensions
- Leginon: a system for fully automated acquisition of 1000 electron micrographs a day.
- Contrast transfer for frozen-hydrated specimens: determination from pairs of defocused images.
- emScope: A Tool Kit for Control and Automation of a Remote Electron Microscope
- The refined crystal structure of cowpea mosaic virus at 2.8 A resolution.
- Toward fully automated high-resolution electron tomography.
- Three-dimensional reconstruction of icosahedral particles--the uncommon line.
- Perforated support foils with pre-defined hole size, shape and arrangement
- Automated microscopy for electron tomography.
- Viewit: a software system for multidimensional biomedical image processing, analysis, and visualization
- Towards automatic electron tomography
Cited by
- Automated image acquisition for single-particle reconstruction using p97 as the biological sample.
- The 17A structure of the 420 kDa lobster clottable protein by single particle reconstruction from cryoelectron micrographs.
- Reconstructing virus structures from nanometer to near-atomic resolutions with cryo-electron microscopy and tomography
- Universal Protection against Influenza Infection by a Multi-Domain Antibody to Influenza Hemagglutinin
- In situ structures of rotavirus polymerase in action and mechanism of mRNA transcription and release
- Automation of image processing in electron crystallography.
- Automated data collection for electron microscopic tomography.
- Proteïnes no estructurals de picornavirus: implicacions funcionals de les estructures cristal·logràfiques de 2B i 3DPOL
- Towards unbiased 3D reconstruction : in single-particle cryo-electron microscopy
- Automatic Virus Identification using TEM : Image Segmentation and Texture Analysis ; Automatisk identifiering av virus med hjälp av transmissionselektronmikroskopi : bildsegmentering och texturanalys
- Electron tomography : methods for three-dimensional visualization of structures in the cell
- Model-based 3-D reconstruction of transparent objects from a single image
- Electron microscopy of integrins.
- New methods for electron tomography
- ATOMIC RESOLUTION CRYO ELECTRON MICROSCOPY OF MACROMOLECULAR COMPLEXES
- Fuzzy inference system as decision-maker to automate cryo-EM data acquisition on a transmission electron microscope
- High-throughput high-resolution cryo-electron crystallography
- Automated cryo-electron microscopy
- Ubp6 deubiquitinase controls conformational dynamics and substrate degradation of the 26S proteasome
- Single particle 3D reconstruction for 2D crystal images of membrane proteins.
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