A robust cell culture system supporting the complete life cycle of hepatitis B virus
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Summary
It is shown that maintaining PEG in culture medium increases the rate of infection by at least one order of magnitude, and, most importantly, promotes virus spread, which can be easily applied to existing cell culture systems to study the complete HBV life cycle, including virus spread.
- Type
- article
- Published
- 2017-11-30
- Cited by
- 79
- References
- 29
- Access
- Open access
- OpenAlex
- https://openalex.org/W2770860859
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:37372617
Keywords
Hepatitis B virus, Virology, Virus, Cell culture, Viral life cycle
References
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Cited by
- Interferon-stimulated gene 20 kDa protein serum levels and clinical outcome of hepatitis B virus-related liver diseases
- Hepatitis B virus genome recycling and de novo secondary infection events maintain stable cccDNA levels
- Robust Human and Murine Hepatocyte Culture Models of Hepatitis B Virus Infection and Replication
- Cell and Animal Models for Studying Hepatitis B Virus Infection and Drug Development
- Efficient long-term amplification of hepatitis B virus isolates after infection of slow proliferating HepG2-NTCP cells.
- The Effect of the Hepatitis B Virus Surface Protein Truncated sC69∗ Mutation on Viral Infectivity and the Host Innate Immune Response
- Hepatitis B Virus–Hepatocyte Interactions and Innate Immune Responses: Experimental Models and Molecular Mechanisms
- Effects of Moloney Leukemia Virus 10 Protein on Hepatitis B Virus Infection and Viral Replication
- Human apolipoprotein E promotes hepatitis B virus infection and production
- RNA Helicase DDX5 Negatively Regulates Wnt Signaling and Hepatocyte Reprogramming in Hepatitis B Virus-related Hepatocellular Carcinoma
- An RNA-based system to study hepatitis B virus replication and select drug-resistance mutations
- The interferon stimulated gene 20 protein (ISG20) is an innate defense antiviral factor that discriminates self versus non-self translation
- An Immortalized Hepatocyte-Like Cell Line (imHC) Accommodated Complete Viral Lifecycle, Viral Persistence Form, cccDNA and Eventual Spreading of a Clinically-Isolated HBV
- Antiviral activity of interferon‐stimulated gene 20, as a putative repressor binding to hepatitis B virus enhancer II and core promoter
- Expansion, in vivo–ex vivo cycling, and genetic manipulation of primary human hepatocytes
- LY6E impairs coronavirus fusion and confers immune control of viral disease
- Public broadly neutralizing antibodies against hepatitis B virus in individuals with elite serologic activity
- The E3 Ubiquitin Ligase TRIM21 Promotes HBV DNA Polymerase Degradation
- In Vitro Systems for Studying Different Genotypes/Sub-Genotypes of Hepatitis B Virus: Strengths and Limitations
- Synchronized infection identifies early rate-limiting steps in the hepatitis B virus life cycle
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