Spinoculation Enhances HBV Infection in NTCP-Reconstituted Hepatocytes
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Summary
A NTCP-complemented HepG2 cell line is established that supports HBV infection, albeit at a low infectivity level following the reported infection procedures, and data suggest that spinoculation could serve as a standard protocol for enhancing the efficiency ofHBV infection in vitro.
- Type
- article
- Published
- 2015-06-12
- Cited by
- 77
- References
- 48
- Access
- Open access
- OpenAlex
- https://openalex.org/W576512939
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:6457104
Keywords
Infectivity, Hepatitis B virus, Virology, Centrifugation, In vitro
References
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- Two distinct types of hepatitis in experimental hepatitis B virus infection.
- Enhancement of infectivity of murine cytomegalovirus in vitro by centrifugal inoculation.
- Alix regulates egress of hepatitis B virus naked capsid particles in an ESCRT‐independent manner
- Infection of a human hepatoma cell line by hepatitis B virus
- The ins and outs of hepatitis C virus entry and assembly
- Cyclosporin A inhibits hepatitis B and hepatitis D virus entry by cyclophilin-independent interference with the NTCP receptor.
- Molecular virology of hepatitis B virus for clinicians.
- High-efficiency retroviral-mediated gene transfer into human and nonhuman primate peripheral blood lymphocytes.
- Spinoculation of heparan sulfate deficient cells enhances HSV-1 entry, but does not abolish the need for essential glycoproteins in viral fusion.
- Increasing the efficiency of virus infectivity assays: small inoculum volumes are as effective as centrifugal enhancement.
- Formation of Hepatitis B Virus Covalently Closed Circular DNA: Removal of Genome-Linked Protein
- Hepatitis B virus infection initiates with a large surface protein-dependent binding to heparan sulfate proteoglycans.
- Evaluation and identification of hepatitis B virus entry inhibitors using HepG2 cells overexpressing a membrane transporter NTCP.
- Cytomegalovirus infectivity: analysis of the phenomenon of centrifugal enhancement of infectivity.
- Centrifugal enhancement of retroviral mediated gene transfer.
- Characterization of the Intracellular Deproteinized Relaxed Circular DNA of Hepatitis B Virus: an Intermediate of Covalently Closed Circular DNA Formation
- Spinoculation Triggers Dynamic Actin and Cofilin Activity That Facilitates HIV-1 Infection of Transformed and Resting CD4 T Cells
- Targeting Hepatitis B Virus With CRISPR/Cas9
- Inhibition of Hepatitis B Virus Replication by the Host Zinc Finger Antiviral Protein
Cited by
- From DCPD to NTCP: The long journey towards identifying a functional hepatitis B virus receptor
- Advances and Challenges in Studying Hepatitis B Virus In Vitro
- HBV culture and infectious systems
- Establishment of an Inducible HBV Stable Cell Line that Expresses cccDNA-dependent Epitope-tagged HBeAg for Screening of cccDNA Modulators
- Unusual Features of Sodium Taurocholate Cotransporting Polypeptide as a Hepatitis B Virus Receptor
- Hepatitis B virus molecular biology and pathogenesis
- Hepatitis B Virus Polymerase Localizes to the Mitochondria, and Its Terminal Protein Domain Contains the Mitochondrial Targeting Signal
- Detection of Hepatitis B Virus Particles Released from Cultured Cells by Particle Gel Assay
- Establishment of a human hepatocellular cell line capable of maintaining long-term replication of hepatitis B virus
- Interferon-inducible ribonuclease ISG20 inhibits hepatitis B virus replication through directly binding to the epsilon stem-loop structure of viral RNA
- Immunopathogenesis of Hepatitis B Virus Infection and Related Complications
- Human Cells as Platform to Produce Gamma-Carboxylated Proteins.
- HBsAg mRNA degradation induced by a dihydroquinolizinone compound depends on the HBV posttranscriptional regulatory element
- A robust cell culture system supporting the complete life cycle of hepatitis B virus
- Generation of hepatocellular cell line capable of supporting the full replication cycle of Hepatitis B Virus
- The role of host DNA ligases in hepadnavirus covalently closed circular DNA formation
- AGK2, A SIRT2 Inhibitor, Inhibits Hepatitis B Virus Replication In Vitro And In Vivo
- Robust Human and Murine Hepatocyte Culture Models of Hepatitis B Virus Infection and Replication
- Hepatitis B Virus–Induced Imbalance of Inflammatory and Antiviral Signaling by Differential Phosphorylation of STAT1 in Human Monocytes
- Hepatitis B Virus Precore Protein p22 Inhibits Alpha Interferon Signaling by Blocking STAT Nuclear Translocation
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