Increasing the efficiency of CRISPR‐Cas9‐VQR precise genome editing in rice
Explore this paper's citation graph
Summary
The modified CRISPR‐Cas9‐VQR system provides a robust toolbox for multiplex genome editing at sites containing noncanonical NGA PAM and significantly increased the editing efficiency of the VQR variant.
- Type
- article
- Published
- 2017-08-05
- Cited by
- 80
- References
- 32
- Access
- Open access
- OpenAlex
- https://openalex.org/W2626278962
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:20690175
Keywords
CRISPR, Biology, Genome editing, Computational biology, Genome
References
- Genetic screens in human cells using the CRISPR/Cas9 system
- Sequence determinants of improved CRISPR sgRNA design
- Rapid Decoding of Sequence-Specific Nuclease-Induced Heterozygous and Biallelic Mutations by Direct Sequencing of PCR Products.
- A QTL for rice grain width and weight encodes a previously unknown RING-type E3 ubiquitin ligase
- A Robust CRISPR/Cas9 System for Convenient, High-Efficiency Multiplex Genome Editing in Monocot and Dicot Plants.
- Targeted genome modification of crop plants using a CRISPR-Cas system
- RNA-Guided Human Genome Engineering via Cas9
- DNA targeting specificity of RNA-guided Cas9 nucleases
- Unbiased detection of off-target cleavage by CRISPR-Cas9 and TALENs using integrase-defective lentiviral vectors
- MONOCULM 3, an ortholog of WUSCHEL in rice, is required for tiller bud formation.
- Multiplex Genome Engineering Using CRISPR/Cas Systems
- Processing-Independent CRISPR RNAs Limit Natural Transformation in Neisseria meningitidis
- High frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells
- Development and Applications of CRISPR-Cas9 for Genome Engineering
- Highly efficient RNA-guided genome editing in human cells via delivery of purified Cas9 ribonucleoproteins
- Structure, expression and promoter activity of two polyubiquitin genes from rice (Oryza sativa L.).
- Isolation of an efficient actin promoter for use in rice transformation.
- Phage Response to CRISPR-Encoded Resistance in Streptococcus thermophilus
- Efficient transformation of rice (Oryza sativa L.) mediated by Agrobacterium and sequence analysis of the boundaries of the T-DNA.
- Enhanced homology-directed human genome engineering by controlled timing of CRISPR/Cas9 delivery
Cited by
- Optimized Cas9 expression systems for highly efficient Arabidopsis genome editing facilitate isolation of complex alleles in a single generation
- Hepatocyte-like cells derived from induced pluripotent stem cells: A versatile tool to understand lipid disorders.
- Robust genome editing of CRISPR-Cas9 at NAG PAMs in rice
- Efficient Knock-in of a Point Mutation in Porcine Fibroblasts Using the CRISPR/Cas9-GMNN Fusion Gene
- CRISPR for Crop Improvement: An Update Review
- Genome Editing in Rice: Recent Advances, Challenges, and Future Implications
- Strengthening the competitiveness of agricultural biotechnology through practical application of gene editing technology
- Clonal seeds in hybrid rice using CRISPR/Cas9
- Clonal seeds from hybrid rice by simultaneous genome engineering of meiosis and fertilization genes
- CRISPR/Cas9-Mediated Adenine Base Editing in Rice Genome
- Development and Application of CRISPR/Cas System in Rice
- Analysis on the international development trends of crop genome editing technology
- The potential application of genome editing by using CRISPR/Cas9, and its engineered and ortholog variants for studying the transcription factors involved in the maintenance of phosphate homeostasis in model plants.
- Increasing Cytosine Base Editing Scope and Efficiency With Engineered Cas9-PmCDA1 Fusions and the Modified sgRNA in Rice
- Increasing fidelity and efficiency by modifying cytidine base-editing systems in rice
- Heat‐shock‐inducible CRISPR/Cas9 system generates heritable mutations in rice
- Data Mining by Pluralistic Approach on CRISPR Gene Editing in Plants
- Improving the efficiency of the CRISPR-Cas12a system with tRNA-crRNA arrays
- Modern Trends in Plant Genome Editing: An Inclusive Review of the CRISPR/Cas9 Toolbox
- Discriminated sgRNAs-based SurroGate System Greatly Enhances the Screening Efficiency of Plant Base-edited Cells.
Related papers
- Elevated expression of exogenous RAD51 enhances the CRISPR/Cas9-mediated genome editing efficiency
- Temperature effect on CRISPR-Cas9 mediated genome editing.
- CRISPR-Cas9 system: A new-fangled dawn in gene editing.
- CRISPR/Cas9 system: A promising technology for the treatment of inherited and neoplastic hematological diseases
- Therapeutic applications of CRISPR RNA-guided genome editing.
- Efficient CRISPR/Cas9 genome editing with low off-target effects in zebrafish
- Chemical transformation mediated CRISPR/Cas9 genome editing in Escherichia coli
- CRISPR/Cas in genome defense and gene editing
- CRISPR/Cas9 Platforms for Genome Editing in Plants: Developments and Applications.
- Virus-Based CRISPR/Cas9 Genome Editing in Plants.