A Robust CRISPR/Cas9 System for Convenient, High-Efficiency Multiplex Genome Editing in Monocot and Dicot Plants.
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Summary
A robust CRISPR/Cas9 vector system, utilizing a plant codon optimized Cas9 gene, for convenient and high-efficiency multiplex genome editing in monocot and dicot plants and provides examples of loss-of-function gene mutations in T0 rice and Arabidopsis plants.
- Type
- article
- Published
- 2015-08-03
- Cited by
- 1,989
- References
- 54
- Access
- Open access
- OpenAlex
- https://openalex.org/W1982802227
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:25184623
Keywords
CRISPR, Biology, Genome editing, Cas9, Arabidopsis
References
- Multiplex and homologous recombination-mediated plant genome editing via guide RNA/Cas9
- TALEN-mediated editing of the Mouse Y Chromosome
- Efficient transformation of rice
- Transient Expression of Virally Delivered Meganuclease In Planta Generates Inherited Genomic Deletions.
- Efficient Virus-Mediated Genome Editing in Plants Using the CRISPR/Cas9 System.
- CRISPR-P: a web tool for synthetic single-guide RNA design of CRISPR-system in plants.
- Rapid Decoding of Sequence-Specific Nuclease-Induced Heterozygous and Biallelic Mutations by Direct Sequencing of PCR Products.
- RNA-guided genome editing in plants using a CRISPR-Cas system.
- Rapid and efficient gene modification in rice and Brachypodium using TALENs.
- Enzymatic assembly of DNA molecules up to several hundred kilobases
- Boosting CRISPR/Cas9 multiplex editing capability with the endogenous tRNA-processing system
- Targeted genome modification of crop plants using a CRISPR-Cas system
- GUIDE-Seq enables genome-wide profiling of off-target cleavage by CRISPR-Cas nucleases
- A plant DNA minipreparation: Version II
- Genome-wide prediction of highly specific guide RNA spacers for CRISPR-Cas9-mediated genome editing in model plants and major crops.
- A One Pot, One Step, Precision Cloning Method with High Throughput Capability
- Both CRISPR/Cas-based nucleases and nickases can be used efficiently for genome engineering in Arabidopsis thaliana.
- Multigeneration analysis reveals the inheritance, specificity, and patterns of CRISPR/Cas-induced gene modifications in Arabidopsis
- Hd3a Protein Is a Mobile Flowering Signal in Rice
- Zinc fingers, TAL effectors, or Cas9-based DNA binding proteins: what's best for targeting desired genome loci?
Cited by
- Emerging Avenues for Utilization of Exotic Germplasm.
- Molecular features of grass allergens and development of biotechnological approaches for allergy prevention.
- Generation of new glutinous rice by CRISPR/Cas9-targeted mutagenesis of the Waxy gene in elite rice varieties
- CRISPR/Cas9‐mediated mutagenesis of lncRNA1459 alters tomato fruit ripening
- A Core Regulatory Pathway Controlling Rice Tiller Angle Mediated by the LAZY1-Dependent Asymmetric Distribution of Auxin[OPEN]
- Reduction of Tomato-Plant Chilling Tolerance by CRISPR-Cas9-Mediated SlCBF1 Mutagenesis.
- A simple and efficient CRISPR/Cas9 platform for induction of single and multiple, heritable mutations in barley (Hordeum vulgare L.)
- Efficient CRISPR/Cas9-mediated Targeted Mutagenesis in Populus in the First Generation
- Egg cell-specific promoter-controlled CRISPR/Cas9 efficiently generates homozygous mutants for multiple target genes in Arabidopsis in a single generation
- DSDecode: A Web-Based Tool for Decoding of Sequencing Chromatograms for Genotyping of Targeted Mutations.
- 基因编辑技术的方法、原理及应用 Methods, Principles and Application of Gene Editing
- A CRISPR/Cas9 Toolbox for Multiplexed Plant Genome Editing and Transcriptional Regulation1[OPEN]
- Use of designer nucleases for targeted gene and genome editing in plants.
- METHOD Open Access
- High-Efficiency Genome Editing in Arabidopsis Using YAO Promoter-Driven CRISPR/Cas9 System.
- Using CRISPR/Cas in three dimensions: towards synthetic plant genomes, transcriptomes and epigenomes.
- Multi-gene engineering in plants with RNA-guided Cas9 nuclease.
- A Simple CRISPR/Cas9 System for Multiplex Genome Editing in Rice.
- Engineering Herbicide-Resistant Rice Plants through CRISPR/Cas9-Mediated Homologous Recombination of Acetolactate Synthase.
- Energy biotechnology in the CRISPR-Cas9 era.
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