Three-Dimensional Cell Culture Systems and Their Applications in Drug Discovery and Cell-Based Biosensors
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Summary
The characteristics of 3D cell culture systems in comparison to the two-dimensional monolayer culture are discussed, focusing on cell growth conditions, cell proliferation, population, and gene and protein expression profiles.
- Type
- review
- Published
- 2014-05-01
- Cited by
- 2,281
- References
- 99
- Access
- Open access
- OpenAlex
- https://openalex.org/W2331628193
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:14831102
Keywords
Drug discovery, Cell, Computational biology, Cell growth, Cell culture
References
- Differences in growth properties of endometrial cancer in three dimensional (3D) culture and 2D cell monolayer.
- Real-time and non-invasive impedimetric monitoring of cell proliferation and chemosensitivity in a perfusion 3D cell culture microfluidic chip.
- The morphologies of breast cancer cell lines in three‐dimensional assays correlate with their profiles of gene expression
- Reversion of the Malignant Phenotype of Human Breast Cells in Three-Dimensional Culture and In Vivo by Integrin Blocking Antibodies
- A novel and simple cell-based detection system with a collagen-encapsulated B-lymphocyte cell line as a biosensor for rapid detection of pathogens and toxins
- A multicellular spheroid-based sensor for anti-cancer therapeutics.
- Importance of Intracellular pH in Determining the Uptake and Efficacy of the Weakly Basic Chemotherapeutic Drug, Doxorubicin
- Human Lung Cancer Cells Grown in an Ex Vivo 3D Lung Model Produce Matrix Metalloproteinases Not Produced in 2D Culture
- Whole-cell biochips for bio-sensing: integration of live cells and inanimate surfaces
- Micropatterns of Matrigel for three-dimensional epithelial cultures.
- A complex 3D human tissue culture system based on mammary stromal cells and silk scaffolds for modeling breast morphogenesis and function
- From Three-Dimensional Cell Culture to Organs-on-Chips
- 3D-biohybrid systems: applications in drug screening.
- Recent advances in three‐dimensional multicellular spheroid culture for biomedical research
- Impact of the 3D Microenvironment on Phenotype, Gene Expression, and EGFR Inhibition of Colorectal Cancer Cell Lines
- Preclinical activity of palifosfamide lysine (ZIO-201) in pediatric sarcomas including oxazaphosphorine-resistant osteosarcoma
- Detection of the osteogenic differentiation of mesenchymal stem cells in 2D and 3D cultures by electrochemical impedance spectroscopy.
- A study of electrochemical biosensor for analysis of three-dimensional (3D) cell culture.
- Development of high throughput microfluidic cell culture chip for perfusion 3-dimensional cell culture-based chemosensitivity assay
- Microcavity array (MCA)-based biosensor chip for functional drug screening of 3D tissue models.
Cited by
- Spatial development of gingival fibroblasts and dental pulp cells: Effect of extracellular matrix.
- Micro/nanoelectrochemical probe and chip devices for evaluation of three-dimensional cultured cells.
- Methods for Expansion of Three-Dimensional Cultures of Human Embryonic Stem Cells Using a Thermoresponsive Polymer
- Detection of DNA Damage Induced by Cerium Dioxide Nanoparticles: From Models to Molecular Mechanism Activated.
- Toward Immunocompetent 3D Skin Models
- Extracellular Matrix as a Regulator of Epidermal Stem Cell Fate
- Hydrogel-incorporating unit in a well: 3D cell culture for high-throughput analysis.
- Luminal MCF-12A & myoepithelial-like Hs 578Bst cells form bilayered acini similar to human breast
- 3D cell-based biosensor for cell viability and drug assessment by 3D electric cell/matrigel-substrate impedance sensing.
- Models of osteoarthritis: the good, the bad and the promising
- Multilayer architecture microfluidic network array for combinatorial drug testing on 3D-cultured cells
- A high-throughput direct fluorescence resonance energy transfer-based assay for analyzing apoptotic proteases using flow cytometry and fluorescence lifetime measurements.
- Challenges and opportunities toward enabling phenotypic screening of complex and 3D cell models.
- Impedance Spectroscopic Characterisation of Porosity in 3D Cell Culture Scaffolds with Different Channel Networks
- Fact or fiction
- Sustained co-delivery of BIO and IGF-1 by a novel hybrid hydrogel system to stimulate endogenous cardiac repair in myocardial infarcted rat hearts
- Towards personalized medicine: chemosensitivity assays of patient lung cancer cell spheroids in a perfused microfluidic platform.
- Screening applications in drug discovery based on microfluidic technology.
- Modeling cancer predisposition: profiling li-fraumeni syndrome patient-derived cell lines using bioinformatics and three-dimensional culture models
- Applications of Computational Modelling and Simulation of Porous Medium in Tissue Engineering
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