A high-throughput direct fluorescence resonance energy transfer-based assay for analyzing apoptotic proteases using flow cytometry and fluorescence lifetime measurements.
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Summary
A high-throughput system to profile cellular populations in terms of time- or dose-dependent responses to apoptotic stimulations because apoptotic inducers are potent anticancer drugs is developed and demonstrates the feasibility of evaluating collective cellular dynamics during apoptosis.
- Type
- article
- Published
- 2015-12-15
- Cited by
- 16
- References
- 50
- Access
- Open access
- OpenAlex
- https://openalex.org/W1815131296
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:21058528
Keywords
Förster resonance energy transfer, Flow cytometry, Apoptosis, Fluorescence, Cell sorting
References
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- Photobleaching recovery and anisotropy decay of green fluorescent protein GFP-S65T in solution and cells: cytoplasmic viscosity probed by green fluorescent protein translational and rotational diffusion.
- Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells
- Purification and cDNA cloning of a second apoptosis-related cysteine protease that cleaves and activates sterol regulatory element binding proteins.
- A Multi-Functional Imaging Approach to High-Content Protein Interaction Screening
- Quantitative comparison of different fluorescent protein couples for fast FRET-FLIM acquisition.
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- Novel calibration method for flow cytometric fluorescence resonance energy transfer measurements between visible fluorescent proteins
- Monitoring of dual bio-molecular events using FRET biosensors based on mTagBFP/sfGFP and mVenus/mKOκ fluorescent protein pairs.
Cited by
- Spectral unmixing plate reader: high-throughput, high-precision FRET assays in living cells
- Novel Fluorescence Tools for the Discovery of Cardiac Calcium Pump Therapeutics
- Development of fluorescent biosensors and inhibitors to probe and target CDK5/p25 in glioblastoma
- Fluorescence lifetime based bioassays
- Overview of Fluorescence Lifetime Measurements in Flow Cytometry.
- Anticancer activity of crude acetone and water extracts of Tulbaghia violacea on human oral cancer cells
- High Throughput Screening Technologies in Biomass Characterization
- Peptide-functionalized upconversion nanoparticles-based FRET sensing platform for Caspase-9 activity detection in vitro and in vivo.
- Phytochemical analysis and biological activities of crude extracts from selected Tulbaghia species
- Enrichment of rare events using a multi-parameter high throughput microfluidic droplet sorter
- Evaluation of Caspase-3 Activity During Apoptosis with Fluorescence Lifetime-Based Cytometry Measurements and Phasor Analyses
- A Review of New High-Throughput Methods Designed for Fluorescence Lifetime Sensing From Cells and Tissues
- Live imaging of apoptotic signaling flow using tunable combinatorial FRET-based bioprobes for cell population analysis of caspase cascades
- Preparation of a Water-Soluble Glycopolymer Bearing Porphyrin Skeletons and Its Biological Properties
- Live Cell Monitoring of Separase Activity, a Key Enzymatic Reaction for Chromosome Segregation, with Chimeric FRET-Based Molecular Sensor upon Cell Cycle Progression
- Fouling-Proof Nanopore for Detecting Caspase-3 in Complex Biological Fluids.
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