Challenges and opportunities toward enabling phenotypic screening of complex and 3D cell models.
Explore this paper's citation graph
Summary
The need for novel chemical sensors to noninvasively quantitate 3D structures in real time is highlighted, and the use of more focused chemical and genomics libraries will enable screening of complex cell models derived from primary and induced pluripotent stem cells.
- Type
- review
- Published
- 2015-04-15
- Cited by
- 18
- References
- 65
- OpenAlex
- https://openalex.org/W2033869308
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:29056292
Keywords
Drug discovery, Induced pluripotent stem cell, Data science, Computational biology, Emerging technologies
References
- The Use of 3-D Cultures for High-Throughput Screening: The Multicellular Spheroid Model
- Miniaturized Three-Dimensional Cancer Model for Drug Evaluation
- Flow-based pipeline for systematic modulation and analysis of 3D tumor microenvironments
- Reversion of the Malignant Phenotype of Human Breast Cells in Three-Dimensional Culture and In Vivo by Integrin Blocking Antibodies
- What's wrong with our cancer models?
- Live Cell Imaging of Caspase Activation for High Content Screening
- Automated neurosphere sorting and plating by the COPAS large particle sorter is a suitable method for high-throughput 3D in vitro applications.
- Matrigel: A complex protein mixture required for optimal growth of cell culture
- Application of Laser-Scanning Fluorescence Microplate Cytometry in High Content Screening
- iPS cell derived neuronal cells for drug discovery.
- Developments in preclinical cancer imaging: innovating the discovery of therapeutics
- Micro instrumentation for characterizing thermoelectric properties of nanomaterials
- A Simple Statistical Parameter for Use in Evaluation and Validation of High Throughput Screening Assays
- Composition and applications of focus libraries to phenotypic assays
- High-Throughput 3D Screening Reveals Differences in Drug Sensitivities between Culture Models of JIMT1 Breast Cancer Cells
- Bright Building Blocks for Chemical Biology
- Phenotypic vs. Target‐Based Drug Discovery for First‐in‐Class Medicines
- Development of Multicellular Tumor Spheroid (MCTS) Culture from Breast Cancer Cell and a High Throughput Screening Method Using the MTT Assay
- Cerebral organoids model human brain development and microcephaly
- Roles of hypoxia‐inducible factor‐1α (HIF‐1α) versus HIF‐2α in the survival of hepatocellular tumor spheroids
Cited by
- Drug screening in 3D in vitro tumor models: overcoming current pitfalls of efficacy read‐outs
- Three‐dimensional cell culture models for anticancer drug screening: Worth the effort?
- Identification of an easy to use 3D culture model to investigate invasion and anticancer drug response in chondrosarcomas
- Transcriptome modeling and phenotypic assays for cancer precision medicine
- How Phenotypic Screening Influenced Drug Discovery: Lessons from Five Years of Practice
- Deciphering Cell Intrinsic Properties: A Key Issue for Robust Organoid Production.
- Functional profiling of microtumors to identify cancer associated fibroblast-derived drug targets
- The Convergence of Stem Cell Technologies and Phenotypic Drug Discovery.
- Resolving Metabolic Heterogeneity in Experimental Models of the Tumor Microenvironment from a Stable Isotope Resolved Metabolomics Perspective
- Modeling of Nanotherapy Response as a Function of the Tumor Microenvironment: Focus on Liver Metastasis
- SERS in 3D cell models: a powerful tool in cancer research
- Spheroids in Cancer Research: Recent Advances and Opportunities
- Chapter 4 Complex High-Content Phenotypic Screening
- Requirements of skin tissue models for high-throughput screening
- Current aspects of organoid technology for biomaterial toxicity analysis.
- In Vitro Assays for Nanoparticle-Cancer Cell Interaction Studies.
- Organoids in Developmental Biology Research and Application
Related papers
- Protein Reporter Bioassay Systems for the Phenotypic Screening of Candidate Drugs: A Mouse Platform for Anti-Aging Drug Screening
- Open Innovation for Phenotypic Drug Discovery: The PD2 Assay Panel
- Troubleshooting and deconvoluting label-free cell phenotypic assays in drug discovery.
- Human Pluripotent Stem Cells as Tools for High-throughput and High-content Screening in Drug Discovery
- Systematic Identification of Pharmacological Targets from Small-Molecule Phenotypic Screens
- Label-free target identification in drug discovery via phenotypic screening.
- Aggregated Compound Biological Signatures Facilitate Phenotypic Drug Discovery and Target Elucidation.
- Phenotype-first screening for the identification of novel drug targets.
- Using in Vitro Evolution and Whole Genome Analysis To Discover Next Generation Targets for Antimalarial Drug Discovery