Genes required for mycobacterial growth defined by high density mutagenesis
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Summary
The use of transposon site hybridization (TraSH) is described to comprehensively identify the genes required by the causative agent, Mycobacterium tuberculosis, for optimal growth, suggesting that the minimal gene set required for survival varies greatly between organisms with different evolutionary histories.
- Type
- article
- Published
- 2003-04-01
- Cited by
- 2,582
- References
- 26
- Access
- Open access
- OpenAlex
- https://openalex.org/W2128482141
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:21383026
Keywords
Biology, Gene, Mycobacterium tuberculosis, Genome, Genetics
References
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Cited by
- Molecular and biochemical characterisation of mycobacterial cell wall drug targets - Lipoarabinomannan
- Detecting selection using a single genome sequence of M. tuberculosis and P. falciparum
- TB tools to tell the tale-molecular genetic methods for mycobacterial research.
- Crystal Structure of Mycobacterium tuberculosis D-3-Phosphoglycerate Dehydrogenase
- Glutamine amidotransferase activity of NAD+ synthetase from Mycobacterium tuberculosis depends on an amino-terminal nitrilase domain.
- Revisiting the Evolution of Mycobacterium bovis
- The serine/threonine kinase PknB of Mycobacterium tuberculosis phosphorylates PBPA, a penicillin-binding protein required for cell division.
- Profiling of Mycobacterium tuberculosis gene expression during human macrophage infection: upregulation of the alternative sigma factor G, a group of transcriptional regulators, and proteins with unknown function.
- Structure and function of Rv0130, a conserved hypothetical protein from Mycobacterium tuberculosis
- A scheme for the analysis of microarray measurements based on a quantitative theoretical framework for bacterial cell growth: application to studies of Mycobacterium tuberculosis.
- Cutinase-like protein-6 of Mycobacterium tuberculosis is recognised in tuberculosis patients and protects mice against pulmonary infection as a single and fusion protein vaccine.
- Energetics of pathogenic bacteria and opportunities for drug development.
- Comparative genomics of NAD(P) biosynthesis and novel antibiotic drug targets
- The roles of pathogen small RNAs
- Novel targets in M. tuberculosis: search for new drugs.
- Occurrence of RD149 and RD152 deletions in Mycobacterium tuberculosis strains from Pakistan.
- Ins and outs of Mycobacterium tuberculosis PPE family in pathogenesis and implications for novel measures against tuberculosis
- Development of metal-chelating inhibitors for the Class II fructose 1,6-bisphosphate (FBP) aldolase.
- Structural and functional studies of the ATP-dependent Clp proteases in cyanobacteria
- The dUTPase Enzyme Is Essential in Mycobacterium smegmatis
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