Production of large unilamellar vesicles by a rapid extrusion procedure: characterization of size distribution, trapped volume and ability to maintain a membrane potential.
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Summary
A technique for the rapid production of large unilamellar vesicles by repeated extrusion under moderate pressures by employing the lipophilic cation methyltriphenylphosphonium (MTPP⁺) and LUVET systems exhibiting a membrane potential in response to a transmembrane Na⁺/K⁺ gradient have been characterized.
- Type
- article
- Published
- 1985-01-10
- Cited by
- 2,074
- References
- 33
- OpenAlex
- https://openalex.org/W2126662679
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:16358991
Keywords
Vesicle, Phosphatidylcholine, Extrusion, Phospholipid, Membrane
References
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- M13 coat protein as a model of membrane assembly
- Introduction to biological membranes
- A rapid and sensitive sub-micro phosphorus determination
- Phospholipid vesicle formation and transmembrane protein incorporation using octyl glucoside.
- Phase transitions in phospholipid model membranes of different curvature.
- Liposomes with a large trapping capacity prepared by freezing and thawing of sonicated phospholipid mixtures.
- Preparation of liposomes of defined size distribution by extrusion through polycarbonate membranes.
- Evidence of bilayer structure and of membrane interactions from X-ray diffraction analysis.
- Thermal analysis of lipids, proteins and biological membranes. A review and summary of some recent studies.
- Uptake of safranine and other lipophilic cations into model membrane systems in response to a membrane potential
- Comparative properties and methods of preparation of lipid vesicles (liposomes).
Cited by
- Highly sequential binding of protein kinase C and related proteins to membranes.
- Selective visualization of choroidal neovascular membranes.
- Phosphatidylethanolamine enhances the concentration-dependent exchange of phospholipids between bilayers.
- On the mechanism of transbilayer transport of phosphatidylglycerol in response to transmembrane pH gradients.
- Quantitation of Ca2+ uptake by Arsenazo III-loaded liposomes.
- N-succinyldioleoylphosphatidylethanolamine: structural preferences in pure and mixed model membranes.
- Distribution of Free and Liposome‐encapsulated Cefoxitin in Experimental Intra‐abdominal Sepsis in Rats
- Destruction of stearic acid nitroxyl radicals mediated by photoexcited merocyanine 540 in liposomal and micellar systems.
- Binding of acylated peptides and fatty acids to phospholipid vesicles: pertinence to myristoylated proteins.
- Peptide hormone-membrane interactions. Intervesicular transfer of lipophilic gastrin derivatives to artificial membranes and their bioactivities.
- Determination by photoreduction of flip-flop kinetics of spin-labeled stearic acids across phospholipid bilayers.
- Stabilized plasmid-lipid particles for regional gene therapy: formulation and transfection properties
- Yersinia enterocolitica type III secretion–translocationsystem: channel formation by secreted Yops
- Biophysical studies and intracellular destabilization of pH-sensitive liposomes
- Specific Binding of Nisin to the Peptidoglycan Precursor Lipid II Combines Pore Formation and Inhibition of Cell Wall Biosynthesis for Potent Antibiotic Activity*
- The curvature and cholesterol content of phospholipid bilayers alter the transbilayer distribution of specific molecular species of phosphatidylethanolamine.
- Parameters influencing the determination of liposome lamellarity by 31P-NMR.
- Relationship between turbidity of lipid vesicle suspensions and particle size.
- Pre‐transmembrane sequence of Ebola glycoprotein
- Liposomal Nanocapsules in Food Science and Agriculture
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