Size determination of sonicated vesicles by freeze‐fracture electron microscopy, using the spray‐freezing method
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Summary
It is found that vesicles of egg lecithin increase in average size with increasing cholesterol concentrations, in agreement with measurements obtained with other techniques.
- Type
- article
- Published
- 1980-04-01
- Cited by
- 54
- References
- 21
- OpenAlex
- https://openalex.org/W1970009144
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:93263608
Keywords
Vesicle, Sonication, Lecithin, Electron microscope, Chemistry
References
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- Integrated stereological and biochemical studies on hepatocytic membranes. III. Relative surface of endoplasmic reticulum membranes in microsomal fractions estimated on freeze-fracture preparations
- The diameters of membrane vesicles fit in geometric series.
- The effect of charge and cholesterol on the size and thickness of sonicated phospholipid vesicles.
- A calorimetric and fluorescent probe study of the gel-liquid crystalline phase transition in small, single-lamellar dipalmitoylphosphatidylcholine vesicles.
- A calorimetric examination of stable and fusing lipid bilayer vesicles.
- Phase transitions in phospholipid model membranes of different curvature.
- Freeze-etching: freezing velocity and crystal size at different locations in samples.
- Geometric packing constraints in egg phosphatidylcholine vesicles.
- Improved cryofixation applicable to freeze etching.
- Vesicles of variable diameter prepared by a modified injection method.
- Outside-inside distributions and sizes of mixed phosphatidylcholine-cholesterol vesicles.
- Cholesterol-phosphatidylcholine interactions in vesicle systems. Implication of vesicle size and proton magnetic resonance line-width changes.
- Methods in membrane biology
- Electron Microscopic Methods in Membrane Biology
Cited by
- Liposomes: preparation, characterization, and preservation.
- Cryofixation: a tool in biological ultrastructural research.
- Lipid requirements for coupled cytochrome oxidase vesicles.
- Spontaneous transfer of ganglioside GM1 from its micelles to lipid vesicles of differing size.
- Spontaneous vesicle formation in aqueous mixtures of single-tailed surfactants.
- Freeze-fracture of lipids and model membrane systems.
- Determination of vesicle size distributions by freeze-fracture electron microscopy.
- Generation of large unilamellar vesicles from long-chain saturated phosphatidylcholines by extrusion technique
- Detection of protein mediated glycosphingolipid clustering by the use of resonance energy transfer between fluorescent labelled lipids. A method established by applying the system ganglioside GM1 and cholera toxin B subunit.
- The freeze fracture ultrastructure of peanut oil and other natural and synthetic triacylglycerol droplets
- Vesicles of variable sizes produced by a rapid extrusion procedure.
- Factors affecting solute entrapment in phospholipid vesicles prepared by the freeze-thaw extrusion method: a possible general method for improving the efficiency of entrapment.
- Generation of multilamellar and unilamellar phospholipid vesicles
- A pulse radiolysis study of the dienyl radical in oxygen-free linoleate solutions: time and linoleate concentration dependence
- Nonuniform lipid distribution in membranes
- A survey of ultra‐rapid cryofixation methods with particular emphasis on applications to freeze‐fracturing, freeze‐etching, and freeze‐substitution
- Weibull distribution of particle sizes obtained by uniform random fragmentation
- Effects of solute concentration on the entrapment of solutes in phospholipid vesicles prepared by freeze-thaw extrusion.
- Contribution of aqueous interphases to the permeability barrier of lipid bilayers for non-electrolytes
- Staining and drying-induced artifacts in electron microscopy of surfactant dispersions
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