Chimeric 16S rRNA sequence formation and detection in Sanger and 454-pyrosequenced PCR amplicons.
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Summary
A new chimera detection tool called Chimera Slayer (CS), which detects chimeras with greater sensitivity than previous methods, performs well on short sequences such as those produced by the 454 Life Sciences (Roche) Genome Sequencer, and can scale to large data sets.
- Type
- article
- Published
- 2011-03-01
- Cited by
- 3,341
- References
- 37
- Access
- Open access
- OpenAlex
- https://openalex.org/W2101120467
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:14848563
Keywords
Biology, Chimera (genetics), Metagenomics, Amplicon, Shotgun sequencing
References
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- QIIME allows analysis of high-throughput community sequencing data
- A general method applicable to the search for similarities in the amino acid sequence of two proteins.
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- Microbial diversity in the deep sea and the underexplored “rare biosphere”
- A phylogeny-driven genomic encyclopaedia of Bacteria and Archaea
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