Wrinkles in the rare biosphere: pyrosequencing errors can lead to artificial inflation of diversity estimates.
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Summary
It is found that 16S rDNA diversity is grossly overestimated unless relatively stringent read quality filtering and low clustering thresholds are applied, and stringent quality-based trimming of 16S pyrotags and clustering threshold no greater than 97% identity should be used to avoid overestimates of the rare biosphere.
- Type
- article
- Published
- 2009-09-01
- Cited by
- 1,286
- References
- 18
- Access
- Open access
- OpenAlex
- https://openalex.org/W1512323415
- Semantic Scholar
- https://api.semanticscholar.org/CorpusID:19870165
Keywords
Biosphere, Pyrosequencing, Diversity (politics), Ecology, Computer science
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